A 3D Cell Culture Organ-on-a-Chip Platform With a Breathable Hemoglobin Analogue Augments and Extends Primary Human Hepatocyte Functions in vitro

remarkable progress in cell culture three-dimensional (3D) technology and organs-on-a-chip has opened doors to recapitulate the complex aspects of human physiology, pathology and drug responses in vitro. Challenges regarding the delivery of oxygen, throughput, multiplexed testing, and experimental complexity which is intended to ensure that the 3D cell culture perfusion of organs-on-a-chip model into routine research tool adopted by academic and industry stakeholders.

To move the field forward, we present a scalable throughput organ-on-a-chip insert a tube system that requires 48 to operate the 3D cell culture models of organ independent statistician. Then, we introduce in-good perfusion to avoid the loss of cell signaling and drug metabolites in an otherwise one-way flow of perfusate. Furthermore, in order to improve the relevance of the model cell culture in 3D in vitro, we address the problem of transport of oxygen by the blood using, for the first time, an analog of hemoglobin breathing to increase gas deliveries cell respiration, as in vivo approximately 98% of the delivery of oxygen to cells occurs through reversible binding hemoglobin.

Next, we show that an increase in cellular metabolic pathways oxygenation shift towards oxidative phosphorylation that contribute to the maintenance of differentiated phenotype in vitro liver. Finally, we show that the activity of the cytochrome family of P450 enzymes of drug metabolism increased and prolonged in hepatocytes human primary cultured in 3D compared with two-dimensional (2D) cell culture gold standard with important consequences for drug metabolism, drug-drug interactions and pharmacokinetic studies in vitro.

Vasculogenesis of Human Dental Pulp Stem Cells Grown in Matrigel with Fully Defined Serum Free Culture Media

Generation of blood vessels is one of the most important challenges in tissue engineering and regeneration. Human cells pulp stem teeth (hDPSCs) are some of the most promising types of stem cells to induce vasculogenesis and angiogenesis because they not only secrete vascular endothelial growth factor (VEGF), but also can differentiate in vitro into both endotheliocytes and pericytes in culture serum-free media.

Additionally, hDPSCs can generate a complete blood vessel that contains both endothelial and mural coating in vivo after transplantation into adult brain. However, many serum-free media used for growth of a supplement containing hDPSCs undisclosed composition. It generates uncertainty as to which component of the appropriate necessary and dumped to the differentiation of vascular hDPSCs, and also block the transfer of basic research findings to clinical cell therapy.

In this work, we designed and tested a new medium that endothelial differentiation to the composition fully defined using standard basal culture medium plus B27 mixture, heparin and growth factors, including VEGF-A165 at different concentrations. We also optimized in vitro Matrigel assay to characterize both hDPSCs ability to differentiate cells of blood vessels and their capacity to produce tubular blood vessel in 3D culture.

Description of serum-free culture medium for the induction of vasculogenesis fully defined using human adult stem cells as a potential highlight relevant innovation for tissue engineering applications. In conclusion, we achieve efficient vasculogenesis start hDPSCs using serum-free culture medium with fully defined composition, which is valid for the purpose of human cell therapy.

Protection of human induced stem cells are pluripotent against shear stresses in suspension culture by Bingham plastic fluid

suspension cultures is an important method used in the industrial preparation of pluripotent stem cells (PSC), for regenerative therapy and drug screening. Generally, suspension culture require agitation to keep the PSC suspended aggregate and to promote mass transfer, but agitation also cause cell damage.

In this study, we investigated the use of Bingham plastic fluid, equipped with a polysaccharide-based polymer, to preserve the PSC of damage to cells in suspension culture. Rheometric analysis shows that the culture medium obtained yield stress and became Bingham plastic fluid, after supplementation with FP003 polymer.

Analysis of growth / death revealed that the formation of aggregates 2 days and 2 days of suspension in a Bingham plastic media improved cell growth and cell death is prevented. After the initial aggregation step, while the strong agitation (120 rpm) of the conventional culture medium resulted in massive cell death, in Bingham plastic fluid we obtain the same growth as normal culture with optimal agitation (90 rpm).

This shows that the Bingham plastic fluid cells are protected from shear stresses in suspension culture and can be used to increase their resilience when developing large scale. This article is protected by copyright. All rights reserved.

Co-Culture System Analysis Enteroids / Colonoids with innate Immune Cells

enteroids human intestine derived from adult stem cells offer the relevant ex vivo system for studying the biological process of the human gut. They create mobile and functional features of the intestinal epithelium of the small intestine (enteroids) or intestine (colonoids) although limited by the lack of relevant cell types that help maintain tissue homeostasis and to respond to external challenges.

In the intestine, innate immune cells interact with the function of epithelial barrier function support, and deploy effector. We have established a co-culture system enteroid / monolayers colonoid and macrophages underlying and polymorphonuclear neutrophils to recapitulate cellular skeleton of human intestinal epithelial niche. Enteroids generated from biopsies or tissue resected from every segment of the intestines of humans and maintained in long-term culture as a three-dimensional structure through supplementation stem cell growth factor. immune cells isolated from human whole blood fresh or frozen peripheral blood mononuclear cells (PBMC).

Monocytes from PBMC differentiated into macrophages by cytokine stimulation prior to co-culture. The method is divided into two main components of the model: (1) generate enteroid / monolayers colonoid and isolating immune cells and (2) the assembly enteroid / colonoid-immune cell co-culture with separate apical and basolateral compartment. Co-culture containing macrophages can be maintained for 48 hours while they neutrophils involved, because of their shorter life span, remain viable for 4 hours.

Enteroid co-cultures of immune-activating several outcome measures, including transepithelial resistance, production of cytokines / chemokines, phenotypic analysis of immune cells, immunofluorescence tissue imaging, protein or mRNA expression, antigen or microbial uptake, and other cellular functions. © 2020 Wiley Periodicals LLC.

Human Cord Blood CD34+ Cells Derived Endothelial Cells
HEC23	Neuromics	500,000+ Cells	EUR 1080

Human Umbilical Cord PrimaCell2: Normal Umbilical Artery Smooth Muscle Cells Growth Medium
9-46107	CHI Scientific	5 x 100 ml	Ask for price

Human Umbilical Cord PrimaCell5: Normal Umbilical Vein Smooth Muscle Cells Growth Medium
9-46110	CHI Scientific	5 x 100 ml	Ask for price

Human Umbilical Cord Tissue Preparation Buffer 1: Normal Umbilical Artery Endothelial Cells
9-80106	CHI Scientific	1 x 100 ml	Ask for price

Human Umbilical Cord Tissue Preparation Buffer 4: Normal Umbilical Vein Endothelial Cells
9-80109	CHI Scientific	1 x 100 ml	Ask for price

Fetal Umbilical Cord Lysate
XBL-10430	ProSci	0.1 mg	EUR 416.75
Description: Fetal human umbilical cord tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The fetal human umbilical cord tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the umbilical cord tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The umbilical cord tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Rat Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Medium
9-25087	CHI Scientific	5 x 100 ml	Ask for price

Mouse Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Medium
9-32091	CHI Scientific	5 x 100 ml	Ask for price

Mouse Umbilical Cord Tissue Preparation Buffer: Normal Umbilical Vein Smooth Muscle Cells
9-80205	CHI Scientific	1 x 100 ml	Ask for price

Rat Umbilical Cord Tissue Preparation Buffer: Normal Umbilical Vein Smooth Muscle Cells
9-80294	CHI Scientific	1 x 100 ml	Ask for price

Human Umbilical Cord Tissue Preparation Buffer 2: Normal Umbilical Artery Smooth Muscle Cells
9-80107	CHI Scientific	1 x 100 ml	Ask for price

Human Umbilical Cord Tissue Preparation Buffer 5: Normal Umbilical Vein Smooth Muscle Cells
9-80110	CHI Scientific	1 x 100 ml	Ask for price

Human Normal Peripheral Blood CD4+/CD25+ Regulatory T Cells (T reg), Cryopreserved
PBCD4-25-C2M	101Bio	-	Ask for price

Human Normal Peripheral Blood CD4+/CD25+ Regulatory T Cells (T reg), Fresh
PBCD4-25-F2M	101Bio	-	Ask for price

Human Cord Blood CD34+ Cells, Cryopreserved, single donor
CBCD34-C0.5M	101Bio	-	Ask for price

Human Cord Blood CD34+ Cells, Cryopreserved, single donorÂ
CBCD34-C1M	101Bio	-	Ask for price

Human Cord Blood CD34+ Cells, Cryopreserved, mixed donorÂ
CBCD34mix-C0.5M	101Bio	-	Ask for price

Human Cord Blood CD34+ Cells, Cryopreserved, mixed donorÂ
CBCD34mix-C1M	101Bio	-	Ask for price

Human Cord Blood CD34+ Cells, Cryopreserved, mixed donorÂ
CBCD34mix-C5M	101Bio	-	Ask for price

anti-CD4 T helper cells
502-A-01mg	Virogen	0,1 mg	EUR 267.5
Description: anti-CD4 T helper cells

anti-CD4 T helper cells
502-A-1000ug	Virogen	1000 ug	EUR 1282.5
Description: anti-CD4 T helper cells

Human Umbilical Cord OptiTDS1: Tissue Dissociation System
4-28106	CHI Scientific	1 Kit	Ask for price

Human Umbilical Cord OptiTDS2: Tissue Dissociation System
4-28107	CHI Scientific	1 Kit	Ask for price

Human Umbilical Cord OptiTDS4: Tissue Dissociation System
4-28109	CHI Scientific	1 Kit	Ask for price

Human Umbilical Cord OptiTDS5: Tissue Dissociation System
4-28110	CHI Scientific	1 Kit	Ask for price

Human Umbilical Cord PrimaCell1: Normal Umbilical Artery Endothelial Cells Growth Supplements with Serum (for 500 ml medium)
9-47106	CHI Scientific	1 Set	Ask for price

Human Umbilical Cord PrimaCell4: Normal Umbilical Vein Endothelial Cells Growth Supplements with Serum (for 500 ml medium)
9-47109	CHI Scientific	1 Set	Ask for price

Human ; Umbilical Vein Endothelial Cells
02-701	Sceti	100ng	EUR 401
Description: The Human ; Umbilical Vein Endothelial Cells is available in Europe and for worldwide shipping via Gentaur.

Human Umbilical Vein Endothelial Cells
HEC01	Neuromics	500,000+ Cells frozen	EUR 440

Human Umbilical Cord PrimaCell2: Normal Umbilical Artery Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)
9-47107	CHI Scientific	1 Set	Ask for price

Human Umbilical Cord PrimaCell5: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)
9-47110	CHI Scientific	1 Set	Ask for price

Mouse Umbilical Cord OptiTDS: Tissue Dissociation System
4-28205	CHI Scientific	1 Kit	Ask for price

Rat Umbilical Cord OptiTDS: Tissue Dissociation System
4-28294	CHI Scientific	1 Kit	Ask for price

Rat Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)
9-26087	CHI Scientific	1 Set	Ask for price

Mouse Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)
9-33091	CHI Scientific	1 Set	Ask for price

Human Peripheral Blood CD3+ T Cells, Cryopreserved
PBCD3-C10M	101Bio	-	Ask for price

Human Peripheral Blood CD3+ T Cells, Cryopreserved
PBCD3-C20M	101Bio	-	Ask for price

Human Peripheral Blood CD3+ T Cells, Fresh
PBCD3-F20M	101Bio	-	Ask for price

Cancer Antigen AFP Antigen (Human Cord Blood)
VAng-Wyb8624-inquire	Creative Biolabs	inquire	Ask for price
Description: Alpha Fetoprotein (66 kDa), Cancer antigen from Human Cord Blood, 1.73 mg/mL.

Human  Cord  Blood  CD34+  Cells (Mixed  Donors)
CBCD34M-05	101Bio	-	Ask for price

Human  Cord  Blood  CD34+  Cells (Mixed  Donors)
CBCD34M-1	101Bio	-	Ask for price

Human  Cord  Blood  CD34+  Cells (Mixed  Donors)
CBCD34M-5	101Bio	-	Ask for price

GFP Expressing Human Umbilical Vein Endothelial Cells
GF01	Neuromics	500,000+ Cells frozen	EUR 1040

Immortalized Human Umbilical Vein Endothelial Cells - SV40
T0014	ABM	1x106 cells / 1.0 ml	Ask for price

Human Umbilical Cord FibrOut1: Fibroblast Inhibitory System (for 500 ml medium)
7-15095	CHI Scientific	1 Kit	Ask for price

Human Umbilical Cord FibrOut2: Fibroblast Inhibitory System (for 500 ml medium)
7-15096	CHI Scientific	1 Kit	Ask for price

Human Umbilical Cord FibrOut4: Fibroblast Inhibitory System (for 500 ml medium)
7-15098	CHI Scientific	1 Kit	Ask for price

Human Umbilical Cord FibrOut5: Fibroblast Inhibitory System (for 500 ml medium)
7-15099	CHI Scientific	1 Kit	Ask for price

Human CD3+ T Cells (Pan T Cells), Cryopreserved
M1299-10	Biovision		Ask for price

Human CD3+ T Cells (Pan T Cells), Cryopreserved
M1299-20	Biovision		Ask for price

Human CD3+ T Cells (Pan T Cells), Cryopreserved
M1299-30	Biovision		Ask for price

Human CD3+ T Cells (Pan T Cells), Cryopreserved
M1299-40	Biovision		Ask for price

Human T-cell surface glycoprotein CD4 (CD4)
1-CSB-YP004935HU1	Cusabio	EUR 430.00 EUR 234.00 EUR 1508.00 EUR 642.00 EUR 1009.00 EUR 291.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human T-cell surface glycoprotein CD4(CD4),partial expressed in Yeast

Human T-cell surface glycoprotein CD4 (CD4)
1-CSB-CF004935HU1	Cusabio	EUR 965.00 EUR 665.00 EUR 715.00	1MG 200ug 500ug
Description: Recombinant Human T-cell surface glycoprotein CD4(CD4),partial expressed in in vitro E.coli expression system

human umbilical vein endothelial cells, Peptide Aptamer, Biotinylated
AP-316-B	Alpha Diagnostics	1 mg	Ask for price

human umbilical vein endothelial cells, Peptide Aptamer, unlabeled
AP-316-U	Alpha Diagnostics	5 mg	Ask for price

Bovine Red Blood Cells
88R-B002	Fitzgerald	20 ml	EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Bovine Red Blood Cells

Chicken Red Blood Cells
88R-C002	Fitzgerald	20 ml	EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Chicken Red Blood Cells

Dog Red Blood Cells
88R-D004	Fitzgerald	10 ml	EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Dog Red Blood Cells

Goat Red Blood Cells
88R-G003	Fitzgerald	20 ml	EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Goat Red Blood Cells

Hamster Red Blood Cells
88R-H001	Fitzgerald	5 ml	EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Hamster Red Blood Cells

Horse Red Blood Cells
88R-H002	Fitzgerald	20 ml	EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Horse Red Blood Cells

Hamster Red Blood Cells
88R-H003	Fitzgerald	10 ml	EUR 489
Description: Washed and freshly prepared 10% suspension of Hamster Red Blood Cells

Mouse Red Blood Cells
88R-M001	Fitzgerald	10 ml	EUR 295
Description: Washed and freshly prepared 10% suspension of Mouse Red Blood Cells

Monkey Red Blood Cells
88R-M003	Fitzgerald	5 x 2 ml	EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Monkey Red Blood Cells

Mouse Red Blood Cells
88R-M004	Fitzgerald	5 x 2 ml	EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Mouse Red Blood Cells

Pig Red Blood Cells
88R-P002	Fitzgerald	20 ml	EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Pig Red Blood Cells

Rabbit Red Blood Cells
88R-R001	Fitzgerald	20 ml	EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Rabbit Red Blood Cells

Rat Red Blood Cells
88R-R002	Fitzgerald	5 x 2.0ml	EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Rat Red Blood Cells

Rat Red Blood Cells
88R-R005	Fitzgerald	25 ml	EUR 327
Description: Washed and freshly prepared 10% suspension of Rat Red Blood Cells

Sheep Red Blood Cells
88R-S001	Fitzgerald	100 ml	EUR 512
Description: Washed and freshly prepared 10% suspension of Sheep Red Blood Cells

Sheep Red Blood Cells
88R-S003	Fitzgerald	20 ml	EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Sheep Red Blood Cells

Sheep Red Blood Cells
88R-S004	Fitzgerald	10 ml	EUR 521
Description: Glutaraldehyde-stabilized freshly prepared albumin sensitised Sheep Red Blood Cells

Sheep Red Blood Cells
88R-S005	Fitzgerald	10 ml	EUR 446
Description: Glutaraldehyde-stabilized freshly prepared tanned Sheep Red Blood Cells

Turkey Red Blood Cells
88R-T001	Fitzgerald	20 ml	EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Turkey Red Blood Cells

Sheep Red Blood Cells
20R-RS001	Fitzgerald	10 ml	EUR 521
Description: Human IgG Senstitized Gluteraldehyde Stabilized Sheep Red Bllood Cells

Unprocessed Human Cord Blood from IRB-approved donors, Fresh
FC-001-F10	101Bio	-	Ask for price

Unprocessed Human Cord Blood from IRB-approved donors, Fresh
FC-001-F100	101Bio	-	Ask for price

Unprocessed Human Cord Blood from IRB-approved donors, Fresh
FC-001-F50	101Bio	-	Ask for price

Unprocessed Human Cord Blood from IRB-approved donors, Fresh
FC-001-F80	101Bio	-	Ask for price

Mouse Umbilical Cord FibrOut: Fibroblast Inhibitory System (for 500 ml medium)
7-15184	CHI Scientific	1 Kit	Ask for price

Rat Umbilical Cord FibrOut: Fibroblast Inhibitory System (for 500 ml medium)
7-15264	CHI Scientific	1 Kit	Ask for price

human umbilical vein endothelial cells, Peptide Aptamer, FITC labelled
AP-316-F	Alpha Diagnostics	1 mg	Ask for price

Human  Peripheral  Blood  CD4+  T  Lymphocytes, Cryopreserved
PBCD4-C10M	101Bio	-	Ask for price

Human  Peripheral  Blood  CD4+  T  Lymphocytes, Fresh
PBCD4-F10M	101Bio	-	Ask for price

Human  Peripheral  Blood  CD4+  T  Lymphocytes, Fresh
PBCD4-F20M	101Bio	-	Ask for price

T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx125313	Abbexa	EUR 495.00 EUR 704.00 EUR 356.00	100 ul 200 ul 50 ul

T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx111482	Abbexa	EUR 732.00 EUR 398.00	150 ul 50 ul

T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx134635	Abbexa	EUR 356.00 EUR 565.00 EUR 300.00	100 ul 200 ul 50 ul

T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx134645	Abbexa	EUR 356.00 EUR 565.00 EUR 300.00	100 ul 200 ul 50 ul

T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx134646	Abbexa	EUR 356.00 EUR 565.00 EUR 300.00	100 ul 200 ul 50 ul

T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx137099	Abbexa	EUR 356.00 EUR 523.00	0.5 mg 1 mg

Basic protocol 1: Nurseries fragment enteroid to Transwells for forming monolayer Alternative Protocol: Nurseries fragment enteroid for forming monolayer using trituration Protocol base 2: Isolation of monocytes, and derivation of immune cells from human peripheral blood basis of Protocol 3: Isolation of neutrophils from human peripheral blood IP base 4: Assembly enteroid / macrophages or enteroid / neutrophil co-culture.