Autologous spermatogonial stem cell transplantation is an experimental method aimed toward restoring fertility in infertile males. Though efficient in animal fashions, in vitro propagation of human spermatogonia previous to transplantation has confirmed to be troublesome. A significant limiting issue is endogenous somatic testicular cell overgrowth throughout long-term tradition.
This makes the tradition each inefficient and necessitates extremely particular cell sorting methods with a view to enrich cultured germ cell fractions previous to transplantation. Right here, we employed RNA-Seq to find out cell kind composition in sorted integrin alpha-6 (ITGA6+) major human testicular cells (n = four donors) cultured for as much as two months, utilizing differential gene expression and cell deconvolution analyses.
Our information and analyses reveal that long-term cultured ITGA6+ testicular cells are composed primarily of cells expressing markers of peritubular myoid cells, (progenitor) Leydig cells, fibroblasts and mesenchymal stromal cells and solely a restricted proportion of spermatogonial cells as in comparison with their uncultured counterparts. These findings present helpful insights into the cell kind composition of cultured human ITGA6+ testicular cells throughout in vitro propagation and should function a foundation for optimizing future cell sorting methods in addition to optimizing the present human testicular cell tradition system for medical use.
3D Cell Tradition of Human Salivary Glands Utilizing Nature-Impressed Purposeful Biomaterials: The Egg Yolk Plasma and Egg White
The egg yolk plasma (EYP)-a translucent fraction of the egg yolk (EY) obtained by centrifugation-was examined as a developmentally encouraging, cost-effective, biomaterial for salivary gland (SG) tissue engineering. To search out optimum incubating circumstances for each the human NS-SV-AC SG acinar cell line and SG fibroblasts, cells had been stained with Reside/Useless®.
The mobile contents of 96-well plates had been analyzed by excessive content material screening picture evaluation. Characteristically, the EYP biomaterial had lipid and protein content material resembling the EY. By itself, the EYP was non-conducive to cell survival. EYP’s pH of 6 primarily contributed to cell loss of life. This was demonstrated by titrating EYP’s pH with completely different concentrations of both industrial cell tradition media, NaOH, or egg white (EW). These components improved SG mesenchymal and epithelial cell survival.
The very best combos had been EYP diluted with (1) 70% industrial medium, (2) 0.02 M NaOH, or (3) 50% EW. Importantly, industrial medium-free progress was obtained with EYP + NaOH or EYP + EW. Moreover, 3D cultures had been obtained because of EW’s gelatinous properties. Right here, the isolation, characterization, and optimization of three EYP-based biomaterial combos are proven; two had been free of business medium or dietary supplements and supported each SG cells’ survival.
Co-Tradition of Main Human Coronary Artery and Inside Thoracic Artery Endothelial Cells Leads to Mutually Helpful Paracrine Interactions
Though saphenous veins (SVs) are generally used as conduits for coronary artery bypass grafting (CABG), inside thoracic artery (ITA) grafts have considerably increased long-term patency. As SVs and ITA endothelial cells (ECs) have a substantial degree of heterogeneity, we advised that synergistic paracrine interactions between CA and ITA ECs (HCAECs and HITAECs, respectively) might clarify the elevated resistance of ITA grafts and adjoining CAs to atherosclerosis and restenosis. On this research, we measured the gene and protein expression of the molecules chargeable for endothelial homeostasis, pro-inflammatory response, and endothelial-to-mesenchymal transition in HCAECs co-cultured with both HITAECs or SV ECs (HSaVECs) for an ascending length.
Upon the co-culture, HCAECs and HITAECs confirmed augmented expression of endothelial nitric oxide synthase (eNOS) and decreased expression of endothelial-to-mesenchymal transition transcription elements Snail and Slug when in comparison with the HCAEC-HSaVEC mannequin. HCAECs co-cultured with HITAECs demonstrated an upregulation of HES1, a grasp regulator of arterial specification, of which the expression was additionally completely induced in HSaVECs co-cultured with HCAECs, suggestive of their arterialisation. As well as, co-culture of HCAECs and HITAECs promoted the discharge of pro-angiogenic molecules. To conclude, co-culture of HCAECs and HITAECs ends in reciprocal and helpful paracrine interactions which may contribute to the higher efficiency of ITA grafts upon CABG.
Rising human trophoblasts in vitro: a overview of the media generally utilized in trophoblast cell tradition
Trophoblasts are distinctive epithelial cells discovered solely within the placenta. It has been attainable to isolate and preserve human trophoblasts in in vitro tradition for a lot of a long time. Throughout this era there have been an enormous array of media and dietary supplements reported for trophoblast tradition and sometimes the explanations for utilizing the media and particular dietary supplements employed in any given laboratory have been misplaced within the ‘mists of time’. After a gradual growth over a few years this area has just lately modified, with the publication of a number of experiences of the isolation, progress and differentiation of human trophoblast stem or stem-like cells.
Human Umbilical Cord PrimaCell4: Normal Umbilical Vein Endothelial Cells |
2-96109 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord Mesenchymal Stem Cells |
HUXUC-01001 |
Cyagen |
1*10^6/vial |
EUR 1159 |
Human Umbilical Cord PrimaCell1: Normal Umbilical Artery Endothelial Cells |
2-96106 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord PrimaCell5: Normal Umbilical Vein Smooth Muscle Cells |
2-96110 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord PrimaCell2: Normal Umbilical Artery Smooth Muscle Cells |
2-96107 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord-Derived Mesenchymal Stem Cells |
cAP-0052 |
Angio Proteomie |
1Frozen Vial |
EUR 594 |
Human Umbilical Cord-derived Mesenchymal Stem Cells |
CSC-00813L |
Creative Bioarray |
One Frozen vial |
Ask for price |
|
Human Umbilical Cord PrimaCell4: Normal Umbilical Vein Endothelial Cells Growth Medium |
9-46109 |
CHI Scientific |
5 x 100 ml |
Ask for price |
Human Umbilical Cord PrimaCell1: Normal Umbilical Artery Endothelial Cells Growth Medium |
9-46106 |
CHI Scientific |
5 x 100 ml |
Ask for price |
Human Umbilical Cord Mesenchymal Stem Cells With GFP |
HUXUC-01101 |
Cyagen |
1*10^6/vial |
EUR 1599 |
Human Umbilical Cord PrimaCell5: Normal Umbilical Vein Smooth Muscle Cells Growth Medium |
9-46110 |
CHI Scientific |
5 x 100 ml |
Ask for price |
Human Umbilical Cord PrimaCell2: Normal Umbilical Artery Smooth Muscle Cells Growth Medium |
9-46107 |
CHI Scientific |
5 x 100 ml |
Ask for price |
Human Umbilical Cord Tissue Preparation Buffer 4: Normal Umbilical Vein Endothelial Cells |
9-80109 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Human Mesenchymal Stem Cells from Umbilical Cord Matrix |
CSC-C1223 |
Creative Bioarray |
One Frozen vial |
Ask for price |
|
Human Umbilical Cord Tissue Preparation Buffer 1: Normal Umbilical Artery Endothelial Cells |
9-80106 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Rat Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells |
2-82587 |
CHI Scientific |
1 Kit |
Ask for price |
Mouse Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells |
2-82091 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord Tissue Preparation Buffer 5: Normal Umbilical Vein Smooth Muscle Cells |
9-80110 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Human Umbilical Cord Tissue Preparation Buffer 2: Normal Umbilical Artery Smooth Muscle Cells |
9-80107 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Human Primary Umbilical Cord-derived Mesenchymal Stem Cells |
T5483 |
ABM |
1x10^6 cells / 1.0 ml |
EUR 1275 |
Complete Medium For Human Umbilical Cord Mesenchymal Stem Cells |
HUXUC-90011 |
Cyagen |
500mL |
EUR 319 |
RFP Expressing Human Umbilical Cord-Derived Mesenchymal Stem Cells |
cAP-0052RFP |
Angio Proteomie |
1Frozen Vial |
EUR 1188 |
RFP Expressing Human Umbilical Cord-Derived Mesenchymal Stem Cells |
RFP11 |
Alphabioregen |
1ml frozen Vial |
EUR 850 |
Serum Free Medium For Human Umbilical Cord Mesenchymal Stem Cells |
HUXUC-90061 |
Cyagen |
500mL |
EUR 399 |
Rat Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Medium |
9-25087 |
CHI Scientific |
5 x 100 ml |
Ask for price |
Serum, Human, Umbilical Cord |
MBS638841-10mL |
MyBiosource |
10mL |
EUR 560 |
Serum, Human, Umbilical Cord |
MBS638841-5x10mL |
MyBiosource |
5x10mL |
EUR 2375 |
Mouse Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Medium |
9-32091 |
CHI Scientific |
5 x 100 ml |
Ask for price |
Rat Umbilical Cord Tissue Preparation Buffer: Normal Umbilical Vein Smooth Muscle Cells |
9-80294 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Umbilical Cord, Human Donor |
MBS170337-INQUIRE |
MyBiosource |
INQUIRE |
Ask for price |
Mouse Umbilical Cord Tissue Preparation Buffer: Normal Umbilical Vein Smooth Muscle Cells |
9-80205 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Immortalized Human Umbilical Cord-Derived Mesenchymal Stem Cells-SV40T |
CSC-I2041Z |
Creative Bioarray |
One Frozen vial |
Ask for price |
Immortalized Bovine Umbilical Cord Endothelial Cells (BUcEC) |
T0834 |
ABM |
1x10^6 cells / 1.0 ml |
Ask for price |
Conditionally Immortalized Human Umbilical Cord-Derived Mesenchymal Stem Cells |
cAP-0062 |
Angio Proteomie |
1Frozen Vial |
EUR 5049 |
Conditionally Immortalized Human Umbilical Cord-Derived Mesenchymal Stem Cells |
ALIMM007 |
Alphabioregen |
1ml frozen Vial |
EUR 0.1 |
Dog Primary Umbilical Cord-derived Mesenchymal Stem Cells |
T5490 |
ABM |
1x10^6 cells / 1.0 ml |
EUR 1145 |
Dog Primary Umbilical Cord-derived Mesenchymal Stem Cells |
T5491 |
ABM |
1x10^6 cells / 1.0 ml |
EUR 1145 |
Immortalized Human Umbilical Cord-Derived Mesenchymal Stem Cells-SV40T+hTERT |
CSC-I2042Z |
Creative Bioarray |
One Frozen vial |
Ask for price |
Human Umbilical Cord PrimaCell1: Normal Umbilical Artery Endothelial Cells Growth Supplements with Serum (for 500 ml medium) |
9-47106 |
CHI Scientific |
1 Set |
Ask for price |
Human Umbilical Cord PrimaCell4: Normal Umbilical Vein Endothelial Cells Growth Supplements with Serum (for 500 ml medium) |
9-47109 |
CHI Scientific |
1 Set |
Ask for price |
Single Donor Human Umbilical Cord |
IRHUSCD |
Innovative research |
each |
EUR 957 |
|
Description: Single Donor Human Umbilical Cord |
Osteogenic Differentiation Medium For Human Umbilical Cord Mesenchymal Stem Cells |
HUXUC-90021 |
Cyagen |
200mL |
EUR 250 |
Adipogenic Differentiation Medium For Human Umbilical Cord Mesenchymal Stem Cells |
HUXUC-90031 |
Cyagen |
200+100mL |
EUR 359 |
Human Umbilical Cord PrimaCell5: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium) |
9-47110 |
CHI Scientific |
1 Set |
Ask for price |
Plasma, Human, Umbilical Cord, Frozen |
MBS634049-10mL |
MyBiosource |
10mL |
EUR 505 |
Plasma, Human, Umbilical Cord, Frozen |
MBS634049-1mL |
MyBiosource |
1mL |
EUR 250 |
Plasma, Human, Umbilical Cord, Frozen |
MBS634049-5x10mL |
MyBiosource |
5x10mL |
EUR 2060 |
Chondrogenic Differentiation Medium For Human Umbilical Cord Mesenchymal Stem Cells |
HUXUC-90041 |
Cyagen |
100mL |
EUR 409 |
Umbilical Cord Serum |
MBS592409-10mL |
MyBiosource |
10mL |
EUR 355 |
Umbilical Cord Serum |
MBS592409-50mL |
MyBiosource |
50mL |
EUR 1350 |
Human Umbilical Cord PrimaCell2: Normal Umbilical Artery Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium) |
9-47107 |
CHI Scientific |
1 Set |
Ask for price |
Immortalized Human Umbilical Cord-Derived Mesenchymal Stem Cells-SV40 (Tet-on) |
CSC-I1909Z |
Creative Bioarray |
One Frozen vial |
Ask for price |
Human Umbilical Cord Fetal Tissue Lysate |
IHUCDFSTL100UG |
Innovative research |
each |
EUR 870 |
|
Description: Human Umbilical Cord Fetal Tissue Lysate |
Human Umbilical Cord Fetal Tissue Lysate |
MBS139153-01mg |
MyBiosource |
0.1mg |
EUR 1145 |
Human Umbilical Cord Fetal Tissue Lysate |
MBS139153-5x01mg |
MyBiosource |
5x0.1mg |
EUR 4960 |
Fetal Umbilical Cord Lysate |
XBL-10430 |
ProSci |
0.1 mg |
EUR 500.1 |
Description: Fetal human umbilical cord tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The fetal human umbilical cord tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the umbilical cord tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The umbilical cord tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Rat Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium) |
9-26087 |
CHI Scientific |
1 Set |
Ask for price |
Mouse Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium) |
9-33091 |
CHI Scientific |
1 Set |
Ask for price |
Human Umbilical Cord OptiTDS1: Tissue Dissociation System |
4-28106 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord OptiTDS2: Tissue Dissociation System |
4-28107 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord OptiTDS4: Tissue Dissociation System |
4-28109 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord OptiTDS5: Tissue Dissociation System |
4-28110 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Vein Endothelial Cells |
ABC-TC3848 |
AcceGen |
1 vial |
Ask for price |
|
Description: HUVEC are isolated from the vein of the umbilical cord and are commonly used for physiological and pharmacological investigations, such as macromolecule transport, blood coagulation, angiogenesis, and fibrinolysis. Human umbilical vein endothelial cells have played a major role as a model system for the study of the regulation of endothelial cell function and the role of the endothelium in the response of the blood vessel wall to stretch, shear forces, and the development of atherosclerotic plaques.Each human umbilical cord vein is individually processed to isolate endothelial cells through collagenase digestion and culture. Frozen HUVEC products are cryopreserved at the end of the primary culture. HUVEC are guaranteed to reach 15 population doublings when cultured with Gentaur's recommended medium.Samples from each donor are tested via PCR to confirm non-reactivity. |
Human ; Umbilical Vein Endothelial Cells |
02-701 |
Sceti |
100ng |
EUR 481.2 |
Description: The Human ; Umbilical Vein Endothelial Cells is available in Europe and for worldwide shipping via Gentaur. |
Human Umbilical Vein Endothelial Cells |
HEC01 |
Neuromics |
500,000+ Cells frozen |
EUR 528 |
Human Umbilical Vein Endothelial Cells |
HUVEC-20001 |
Cyagen |
1*10^6/vial |
EUR 1100 |
Human Umbilical Vein Endothelial Cells |
CSC-C1578 |
Creative Bioarray |
One Frozen vial |
Ask for price |
Human Umbilical Vein Endothelial Cells |
MBS556003-INQUIRE |
MyBiosource |
INQUIRE |
Ask for price |
Human Umbilical Artery Endothelial Cells |
ABC-TC3841 |
AcceGen |
1 vial |
Ask for price |
Description: Gentaur endothelial cells from large vessels are available from different locations, e.g. umbilical vein, umbilical artery, aorta, coronary artery, pulmonary artery, and saphenous vein. In addition, Gentaur offers HUVEC isolated in standard Endothelial Cell Growth Medium and also in Endothelial Cell Growth Medium 2. The cells are supplied either from single donors or from pooled donors (from up to four different umbilical cords). Furthermore, HUVEC are available which are prescreened for VEGF (vascular endothelial cell growth factor) response. Shortly after isolation, Gentaur Human Endothelial Cells from large vessels are cryopreserved at passage 1 or passage 2 (see page 5) using Gentaur's proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing.Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur. |
Human Umbilical Vein Smooth Muscle Cells |
ABC-TC3857 |
AcceGen |
1 vial |
Ask for price |
|
Description: Vascular smooth muscle cell is the cellular substate of most significant arterial disease. The increased growth potential of vascular smooth muscle cells represents one of the crucial anomalies responsible for the development of essential vascular diseases. New studies demonstrate that vascular smooth muscle cells express ICAM-1 and VCAM-1, which may contribute to the inflammatory reaction in the vascular wall and may actively be involved in the progression and stability of vascular disease. In vitro culture of human VSMC as a model of vascular research played a critical role and continues providing information in the pharmacology and the therapy of vascular diseases.HUVSMC from Gentaur Research Laboratories are isolated from human umbilical veins. HUVSMC are cryopreserved at the end of secondary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HUVSMC are characterized by immunofluorescent method with antibodies to ?-smooth muscle actin and desmin. HUVSMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HUVSMC are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories. |
Human Cord Blood CD19+ B Cells |
ABC-TC3368 |
AcceGen |
1 vial |
Ask for price |
|
Description: Cord Blood-CD19+ B cells are positively isolated from mononuclear cells, using a direct immunomagnetic CD19 MicroBeads labeling system |
Human Cord Blood CD19+ B Cells |
CBCD19-C2.5M |
101Bio |
2.5 million |
EUR 906 |
Human Cord Blood CD19+ B Cells |
CBCD19-C5M |
101Bio |
5 million |
EUR 1078.8 |
Human Cord Blood CD19+ B Cells |
CBCD19-F5M |
101Bio |
5 million |
EUR 1198.8 |
Human Umbilical Artery Smooth Muscle Cells |
ABC-TC3843 |
AcceGen |
1 vial |
Ask for price |
Description: Smooth muscle tissue is found in the tunica media layer of large and small blood vessels and in the walls of hollow organs like the bladder and the uterus. Smooth Muscle Cells (SMC) possess all the same types of filaments, but, depending on the tissue of origin, differ significantly in mechanical and physiological properties. Therefore, Gentaur offers a range of Human Smooth Muscle Cells produced at Gentaur's cell culture facility from normal human tissue of different origins. The cells are isolated from the aorta, the coronary artery, the pulmonary artery, the umbilical artery,the trachea, the bronchi, the bladder,the prostate, and the uterus. Shortly after isolation, all Gentaur Human Smooth Muscle Cells are cryopreserved at passage 2 (P2) using Gentaur's proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing.Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur. |
Human Umbilical Vein Endothelial Cells, Pooled |
ABC-TC3849 |
AcceGen |
1 vial |
Ask for price |
|
Description: The cells are cryopreserved in the second passage to ensure the highest viability and plating efficiency. Primary pooled HUVECs are derived from 10 individual donors, minimizing the lot-to-lot variability associated with cells derived from single donors. |
Human Umbilical Vein Endothelial Cells (HUVECs) |
cAP-0001 |
Angio Proteomie |
1Frozen Vial |
EUR 217.8 |
Human Umbilical Vein Endothelial Cells, Neonatal |
ABC-TC3853 |
AcceGen |
1 vial |
Ask for price |
|
Description: Human Umbilical Vein Endothelial Cells (HUVEC) are isolated from normal human umbilical vein. They are cryopreserved at the end of primary culture and can be cultured and propagated at least 16 population doublings. |
Human Umbilical Artery Endothelial Cells (HUAECs) |
cAP-0113 |
Angio Proteomie |
1Frozen Vial |
EUR 396 |
Human Primary Umbilical Vein Endothelial Cells |
T4098 |
ABM |
5x10^5 cells / 1.0 ml |
Ask for price |
Human Primary Umbilical Vein Endothelial Cells |
T5482 |
ABM |
1x10^6 cells / 1.0 ml |
EUR 765 |
Human Umbilical Artery Endothelial Cells, Neonatal |
ABC-TC3842 |
AcceGen |
1 vial |
Ask for price |
|
Description: Human Umbilical Artery Endothelial Cells (HUAEC) are isolated from normal human umbilical arteries. They are cryopreserved at second passage and can be cultured and propagated at least 15 population doublings. |
HighQC™ Human Mesenchymal Stem Cell (Umbilical Cord Matrix/ Cell Pellet In RNAlater) |
ABC-SC0038T |
AcceGen |
1 vial |
Ask for price |
Description: Mesenchymal Stem Cells (MSC), also termed Mesenchymal Stromal Cells, are multipotent cells that can differentiate into a variety of cell types and have the capacity for self renewal. MSC have been shown to differentiate in vitro or in vivo into adipocytes, chondrocytes, osteoblasts, myocytes, neurons, hepatocytes, and pancreatic islet cells. Optimized Gentaur media are available to support both the growth of MSC and their differentiation into several different lineages. Recent experiments suggest that differentiation capabilities into diverse cell types vary between MSC of different origin.Gentaur hMSC are harvested from normal human adipose tissue, bone marrow, and umbilical cord matrix (Wharton's jelly) of individual donors.The cells are tested for their ability to differentiate in vitro into adipocytes, chondrocytes, and osteoblasts. Our hMSC show a verified marker expression profile that complies with ISCT* recommendations, providing well characterized cells. |
Human Primary Umbilical Artery Endothelial Cells |
T4099 |
ABM |
5x10^5 cells / 1.0 ml |
Ask for price |
HighQC™ RFP-Human Umbilical Cord-Derived Mesenchymal Stem Cell |
ABC-FC0048 |
AcceGen |
1 vial |
Ask for price |
Description: HUcMSCs are isolated from umbilical cord matrix (Wharton's Jelly) and demonstrated with spindle-shaped-, and fibroblast-like cells. RFP-HUcMSCs are puromycin resistant HUcMSCs after trandsfected with lentiviruses expressing RFP under the control of CMV protomer. Each lot of RFP-HUcMSCs is tested to ensure the cells can be passaged at least three times (i.e., approximately 9 to 10 population doublings) after thaw in complete growth media (Mesenchymal Stem Cell Growth Medium (MSCGM)), when cultured following the detailed protocol described below. |
Innovative Grade US Origin Bovine Umbilical Cord |
IGBOCDF |
Innovative research |
each |
EUR 210 |
|
Description: Innovative Grade US Origin Bovine Umbilical Cord |
Innovative Grade US Origin Bovine Umbilical Cord |
IGBOCDP |
Innovative research |
each |
EUR 210 |
|
Description: Innovative Grade US Origin Bovine Umbilical Cord |
Innovative Grade US Origin Bovine Umbilical Cord |
IGBOCDS |
Innovative research |
each |
EUR 133 |
|
Description: Innovative Grade US Origin Bovine Umbilical Cord |
Innovative Grade US Origin Bovine Umbilical Cord |
IGBOCDZ |
Innovative research |
each |
EUR 133 |
|
Description: Innovative Grade US Origin Bovine Umbilical Cord |
Immortalized Human Umbilical Vein Endothelial Cells |
CSC-I9038L |
Creative Bioarray |
One Frozen vial |
Ask for price |
Innovative Grade US Origin Porcine Umbilical Cord |
IGPCCDF |
Innovative research |
each |
EUR 133 |
|
Description: Innovative Grade US Origin Porcine Umbilical Cord |
Innovative Grade US Origin Porcine Umbilical Cord |
IGPCCDP |
Innovative research |
each |
EUR 210 |
|
Description: Innovative Grade US Origin Porcine Umbilical Cord |
Innovative Grade US Origin Porcine Umbilical Cord |
IGPCCDS |
Innovative research |
each |
EUR 210 |
|
Description: Innovative Grade US Origin Porcine Umbilical Cord |
Innovative Grade US Origin Porcine Umbilical Cord |
IGPCCDZ |
Innovative research |
each |
EUR 133 |
|
Description: Innovative Grade US Origin Porcine Umbilical Cord |
Human Umbilical Vein Smooth Muscle Cells, Neonatal |
ABC-TC3858 |
AcceGen |
1 vial |
Ask for price |
|
Description: Human umbilical vein smooth muscle cells (HUVSMC) are derived from the umbilical vein. They are cryopreserved at second passage. HUVSMC can be cultured and propagated 6 population doublings. |
Human Primary Umbilical Vein Smooth Muscle Cells |
T4101 |
ABM |
5x10^5 cells / 1.0 ml |
EUR 1375 |
Human Umbilical Vein Endothelial Cells, Pooled Donor |
ABC-TC3854 |
AcceGen |
1 vial |
Ask for price |
|
Description: HUVEC-Umbilical Vein Endothelial Cells are available as single donor or pooled varieties. |
Human Umbilical Vein Endothelial Cells, Single Donor |
ABC-TC3855 |
AcceGen |
1 vial |
Ask for price |
Description: Gentaur endothelial cells from large vessels are available from different locations, e.g. umbilical vein, umbilical artery, aorta, coronary artery, pulmonary artery, and saphenous vein. In addition, Gentaur offers HUVEC isolated in standard Endothelial Cell Growth Medium and also in Endothelial Cell Growth Medium 2. The cells are supplied either from single donors or from pooled donors (from up to four different umbilical cords). Furthermore, HUVEC are available which are prescreened for VEGF (vascular endothelial cell growth factor) response. Shortly after isolation, Gentaur Human Endothelial Cells from large vessels are cryopreserved at passage 1 or passage 2 (see page 5) using Gentaur's proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing.Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur. |
Human Umbilical Artery Smooth Muscle Cells, Neonatal |
ABC-TC4269 |
AcceGen |
1 vial |
Ask for price |
|
Description: Human Umbilical Artery Smooth Muscle Cells (HUASMC) are isolated from normal human umbilical arteries. They are cryopreserved at second passage and can be cultured and propagated at least 16 population doublings. Umbilical artery smooth muscle cells have |
Rat Umbilical Cord OptiTDS: Tissue Dissociation System |
4-28294 |
CHI Scientific |
1 Kit |
Ask for price |
Human Primary Umbilical Artery Smooth Muscle Cells |
T4100 |
ABM |
5x10^5 cells / 1.0 ml |
Ask for price |
Human Umbilical Vein Endothelial Cells, Pre-screened |
ABC-TC3850 |
AcceGen |
1 vial |
Ask for price |
Description: Gentaur endothelial cells from large vessels are available from different locations, e.g. umbilical vein, umbilical artery, aorta, coronary artery, pulmonary artery, and saphenous vein. In addition, Gentaur offers HUVEC isolated in standard Endothelial Cell Growth Medium and also in Endothelial Cell Growth Medium 2. The cells are supplied either from single donors or from pooled donors (from up to four different umbilical cords). Furthermore, HUVEC are available which are prescreened for VEGF (vascular endothelial cell growth factor) response. Shortly after isolation, Gentaur Human Endothelial Cells from large vessels are cryopreserved at passage 1 or passage 2 (see page 5) using Gentaur's proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing.Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur. |
GFP Expressing Human Umbilical Vein Endothelial Cells |
GF01 |
Neuromics |
500,000+ Cells frozen |
EUR 1248 |
GFP Expressing Human Umbilical Vein Endothelial Cells |
cAP-0001GFP |
Angio Proteomie |
1Frozen Vial |
EUR 643.5 |
RFP Expressing Human Umbilical Vein Endothelial Cells |
cAP-0001RFP |
Angio Proteomie |
1Frozen Vial |
EUR 643.5 |
GFP Expressing Human Umbilical Vein Endothelial Cells |
MBS555999-INQUIRE |
MyBiosource |
INQUIRE |
Ask for price |
Mouse Umbilical Cord OptiTDS: Tissue Dissociation System |
4-28205 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord FibrOut1: Fibroblast Inhibitory System (for 500 ml medium) |
7-15095 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord FibrOut2: Fibroblast Inhibitory System (for 500 ml medium) |
7-15096 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord FibrOut4: Fibroblast Inhibitory System (for 500 ml medium) |
7-15098 |
CHI Scientific |
1 Kit |
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Human Umbilical Cord FibrOut5: Fibroblast Inhibitory System (for 500 ml medium) |
7-15099 |
CHI Scientific |
1 Kit |
Ask for price |
Immortalized Human Umbilical Vein Endothelial Cells - SV40 |
T0014 |
ABM |
1x106 cells / 1.0 ml |
EUR 1085 |
Human Umbilical Vein Endothelial Cells, 10-Donor Pool |
ABC-TC3852 |
AcceGen |
1 vial |
Ask for price |
|
Description: HUVEC10-Donor Pools, when grown in LIVas Medium, provide an ideal low-serum culture model, with or without human VEGF, for the study of angiogenesis, atherosclerosis or vascular biology without the concern of atypical results from a single donor. HUVEC 10-Donor Pools are cryopreserved as secondary cells to ensure the highest viability and plating efficiency.Cell Features:HUVEC are isolated from human umbilical cords, cultured on plastic and cryopreserved as primary cells.HAoEC and HUVEC 10-Donor Pool are isolated from human aorta or umbilical cord, cultured on plastic and cryopreserved as secondary cells.HPAEC are isolated from human pulmonary artery, cultured on plastic and cryopreserved as secondary cells.HCAEC are isolated from human coronary arteries, cultured on plastic and cryopreserved as tertiary cells.Human endothelial cells can be grown in low serum (2%) medium without phenol red or antimicrobials when cultured in VascuLife medium.Culture human endothelial cells with or without VEGF. human endothelial cells are extensively tested to meet quality standards and exhibit optimal performance.Gentaur guarantees performance and quality. |
GCaMP3-Expressing Human Umbilical Vein Endothelial Cells |
cAP-0001-GCaMP3 |
Angio Proteomie |
1Frozen Vial |
EUR 643.5 |
GCaMP3-Expressing Human Umbilical Vein Endothelial Cells |
ALHC069 |
Alphabioregen |
1ml frozen Vial |
EUR 0.2 |
GFP-Human Umbilical Vein Endothelial Cells |
ABC-FC0001 |
AcceGen |
1 vial |
Ask for price |
|
Description: HUVECs were infected with GFP- expressing Lentiviral particles @ passage 1. Puromycin resistant GFP-HUVECs were selected and shipped in frozen vials (cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum of 18 population doubling levels when cultured following the protocols described below. |
RFP-Human Umbilical Vein Endothelial Cells |
ABC-FC0004 |
AcceGen |
1 vial |
Ask for price |
|
Description: HUVECs were infected with RFP- expressing Lentiviral particles @ passage 1. Zeocin resistant RPF-HUVECs were selected and shipped in frozen vials (cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum of 18 population doubling levels when cultured following the protocols described below). |
GCaMP6m-Expressing Human Umbilical Vein Endothelial Cells |
cAP-0001-GCaMP6m |
Angio Proteomie |
1Frozen Vial |
EUR 643.5 |
Immortalized Human Umbilical Vein Endothelial Cells-hTERT |
CSC-I9007L |
Creative Bioarray |
One Frozen vial |
Ask for price |
GCaMP6m-Expressing Human Umbilical Vein Endothelial Cells |
ALHC070 |
Alphabioregen |
1ml frozen Vial |
EUR 0.2 |
GFP-Human Umbilical Artery Endothelial Cells |
ABC-FC0021 |
AcceGen |
1 vial |
Ask for price |
|
Description: HUAECs are pooled cells isolated from normal human umbilical artery. GFP-HUAECs were selected from HUAECs infected with GFP-expressing lentiviruse with Puromycin and the cells are shipped in frozen vials (the cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 20 when cultured following the detailed protocol. |
RFP-Human Umbilical Artery Endothelial Cells |
ABC-FC0022 |
AcceGen |
1 vial |
Ask for price |
|
Description: HUAECs are pooled cells isolated from normal human umbilical artery. RFP-HUAECs were selected from HUAECs infected with RFP-expressing lentiviruses with Zyocin and the cells are shipped in frozen vials (the cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 20 when cultured following the detailed protocol. |
Complete Medium For Human Umbilical Vein Endothelial Cells |
HUVEC-90011 |
Cyagen |
500mL |
EUR 319 |
Mitochondria GFP Tag Human Umbilical Vein Endothelial Cells |
ABC-FC0002 |
AcceGen |
1 vial |
Ask for price |
|
Description: HUVECs were infected with Lentiviral particles expressing GFP-Tagged Cytochrome C oxidase VIII @ passage 1. Puromycin resistant Mitochondria-GFP-HUVECs were selected and shipped in frozen vials (cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum of 18 population doubling levels when cultured following the protocols described below). |
Mitochondria GFP Tag Human Umbilical Vein Endothelial Cells |
GF16 |
Alphabioregen |
1ml frozen Vial |
EUR 850 |
Mitochondria GFP Tag Human Umbilical Vein Endothelial Cells |
cAP-0001GFP-Mitochon |
Angio Proteomie |
1Frozen Vial |
EUR 792 |
CORNING®HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS, HUVEC-2 CELLS (>5X105 CELLS), 1 CRYOVIAL/PACK |
354151 |
CORNING |
1/pk |
EUR 465.6 |
Description: Permeable Support - DL; Coated Transwells - DL |
Immortalized Human Umbilical Vein Endothelial Cells (SV40)-GFP |
CSC-I2045Z |
Creative Bioarray |
One Frozen vial |
Ask for price |
Immortalized Human Umbilical Vein Endothelial Cells (SV40)-RFP |
CSC-I2046Z |
Creative Bioarray |
One Frozen vial |
Ask for price |
Immortalized Human Umbilical Vein Endothelial Cells (PDL8 HUVEC) |
T0725 |
ABM |
1x10^6 cells / 1.0 ml |
EUR 1085 |
human umbilical vein endothelial cells, Peptide Aptamer, unlabeled |
AP-316-U |
Alpha Diagnostics |
5 mg |
Ask for price |
Exosome-GFP Expressing Human Umbilical Vein Endothelial Cells |
cAP-0001GFP-Exos |
Angio Proteomie |
1Frozen Vial |
EUR 792 |
Human Pre-screened Umbilical Vein Endothelial Cells, Neonatal |
ABC-TC3763 |
AcceGen |
1 vial |
Ask for price |
|
Description: Pre-screened for endothelial properties. Human Umbilical Vein Endothelial Cells (HUVEC) are isolated from normal human umbilical vein. They are cryopreserved at the end of primary culture and can be cultured and propagated at least 16 population doubling. |
Plasma Membrane GFP Tag Human Umbilical Vein Endothelial Cells |
ABC-FC0003 |
AcceGen |
1 vial |
Ask for price |
|
Description: HUVECs were infected with Lentiviral particles expressing GFP-Tagged neuromodulin @ passage 1. Puromycin resistant PM-GFP-HUVECs were selected and shipped in frozen vials (cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum of 18 population doubling levels when cultured following the protocols described below). |
Plasma Membrane GFP Tag Human Umbilical Vein Endothelial Cells |
GF17 |
Alphabioregen |
1ml frozen Vial |
EUR 850 |
Plasma Membrane GFP Tag Human Umbilical Vein Endothelial Cells |
cAP-0001GFP-PM |
Angio Proteomie |
1Frozen Vial |
EUR 792 |
human umbilical vein endothelial cells, Peptide Aptamer, Biotinylated |
AP-316-B |
Alpha Diagnostics |
1 mg |
Ask for price |
GFP-Human Umbilical Artery Endothelial Cells (HUAECs) |
cAP-0113GFP |
Angio Proteomie |
1Frozen Vial |
EUR 643.5 |
RFP-Human Umbilical Artery Endothelial Cells (HUAECs) |
cAP-0113RFP |
Angio Proteomie |
1Frozen Vial |
EUR 643.5 |
GFP-Expressing Human Umbilical Vein Microvascular Endothelial cells |
GF1 |
Alphabioregen |
1ml frozen Vial |
EUR 620 |
human umbilical vein endothelial cells, Peptide Aptamer, FITC labelled |
AP-316-F |
Alpha Diagnostics |
1 mg |
Ask for price |
UASMC Umbilical Artery Smooth Muscle cells, cryo |
LOCC-2579 |
Westburg |
each |
EUR 1474.2 |
Human Umbilical Vein Endothelial Cells (HUVECs) Expressing VE-CAD-GFP |
cAP-0001VECAD-GFP |
Angio Proteomie |
1Frozen Vial |
EUR 742.5 |
Rat Umbilical Cord FibrOut: Fibroblast Inhibitory System (for 500 ml medium) |
7-15264 |
CHI Scientific |
1 Kit |
Ask for price |
Mouse Umbilical Cord FibrOut: Fibroblast Inhibitory System (for 500 ml medium) |
7-15184 |
CHI Scientific |
1 Kit |
Ask for price |
VEGF-stimulated human umbilical vein endothelial cells, Peptide Aptamer, unlabeled |
AP-339-U |
Alpha Diagnostics |
5 mg |
Ask for price |
VEGF-stimulated human umbilical vein endothelial cells, Peptide Aptamer, Biotinylated |
AP-339-B |
Alpha Diagnostics |
1 mg |
Ask for price |
Human Umbilical Vein Endothelial Cells (HUVECs) with Aequorin Expressed in Mitochondria |
cAP-0001-AEQ-Mito |
Angio Proteomie |
1Frozen Vial |
EUR 990 |
VEGF-stimulated human umbilical vein endothelial cells, Peptide Aptamer, FITC labelled |
AP-339-F |
Alpha Diagnostics |
1 mg |
Ask for price |
Human Umbilical Vein Endothelial |
ABC-TC3997 |
AcceGen |
1 vial |
Ask for price |
|
Description: Gentaur's normal Human Umbilical Vein Endothelial Cells (HUVEC), when grown in Gentaur's LIVas Medium, provides an ideal low serum culture model, with or without human VEGF, for the study of angiogenesis, atherosclerosis or vascular biology. Gentaur's HUVEC are cryopreserved as primary cells to ensure the highest viability and plating efficiency. Our HUVEC are quality tested in LIVas 2% serum medium to ensure optimal reduced-serum growth over a period of at least 15 population doublings at rates equal to or greater than serum-supplemented medium. Cell Features:HUVEC are isolated from human umbilical cords, cultured on plastic and cryopreserved as primary cells.HAoEC and HUVEC 10-Donor Pool are isolated from human aorta or umbilical cord, cultured on plastic and cryopreserved as secondary cells.HPAEC are isolated from human pulmonary artery, cultured on plastic and cryopreserved as secondary cells.HCAEC are isolated from human coronary arteries, cultured on plastic and cryopreserved as tertiary cells.Human endothelial cells can be grown in low serum (2%) medium without phenol red or antimicrobials when cultured in VascuLife medium.Culture human endothelial cells with or without VEGF.Gentaur's human endothelial cells are extensively tested to meet quality standards and exhibit optimal performance.Gentaur guarantees performance and quality. |
Human Cord Blood CD56+ NK Cells |
ABC-TC3378 |
AcceGen |
1 vial |
Ask for price |
|
Description: Cord Blood CD56+ Natural Killer (NK) Cells are positively isolated from Mononuclear Cells using a direct immunomagnetic CD56 MicroBeads labeling system. |
Human Cord Blood CD56+ Nk Cells |
CBCD56-C1M |
101Bio |
1 million |
EUR 886.8 |
Human Cord Blood CD56+ Nk Cells |
CBCD56-F1M |
101Bio |
1 million |
EUR 994.8 |
Human Cord Blood CD133+ Cells, Mixed |
ABC-TC3366 |
AcceGen |
1 vial |
Ask for price |
|
Description: Cord Blood-CD133+ Stem/Progenitor cells are positively isolated using a direct immunomagnetic CD133 MicroBead labeling system. The majority of CD133+ stem/progenitor cells express the CD34 cell marker as well. Cord Blood-CD133+ Cells/Mixed indicates that multiple donors are used to obtain the cord blood CD133+ cells |
Immortalized Human Umbilical Vein Endothelial Cell Line |
ABI-TC051D |
AcceGen |
1 vial |
Ask for price |
Description: The endothelium is the thin layer of cells that lines the interior surface of blood vessels and lymphatic vessels, forming an interface between circulating blood or lymph in the lumen and the rest of the vessel wall. Gentaur currently offers human endothelial cells isolated from the vein of the human umbilical cord (HUVEC). |
Human Cord Blood CD34+ Cells, Mixed |
ABC-TC3369 |
AcceGen |
1 vial |
Ask for price |
|
Description: Cord Blood-CD34+ Stem/Progenitor cells are positively isolated using a direct immunomagnetic CD34 MicroBead labeling system.Cord Blood-CD34+ Cells/Mixed indicates that multiple donors are pooled to obtain the cord blood CD34+ cells. |
Human Cord Blood CD34+ Cells, Single |
ABC-TC3371 |
AcceGen |
1 vial |
Ask for price |
|
Description: Cord Blood-CD34+ Stem/Progenitor cells are positively isolated using a direct immunomagnetic CD34 MicroBead labeling system. Cord Blood-CD34+ Cells/Single indicates that only one donor is used to obtain the cord blood CD34+ cells. |
Human Cord Blood CD34+ Cells Derived Endothelial Cells |
HEC23 |
Neuromics |
500,000+ Cells |
EUR 1296 |
Human Cord Blood CD34+ Cells Derived Endothelial Cells |
cAP-0024 |
Angio Proteomie |
1Frozen Vial |
EUR 693 |
Human Cord Blood CD34+ Cells Derived Endothelial Cells |
MBS556026-INQUIRE |
MyBiosource |
INQUIRE |
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This advance was made largely due to a better understanding of the molecular pathways that management human trophoblasts and availability of media dietary supplements that can be utilized to govern these pathways. We now have searched the literature and right here summarise lots of the completely different media and dietary supplements and describe how and why they had been developed and are used to tradition human trophoblasts.