A 3D Cell Culture Organ-on-a-Chip Platform With a Breathable Hemoglobin Analogue Augments and Extends Primary Human Hepatocyte Functions in vitro

remarkable progress in cell culture three-dimensional (3D) technology and organs-on-a-chip has opened doors to recapitulate the complex aspects of human physiology, pathology and drug responses in vitro. Challenges regarding the delivery of oxygen, throughput, multiplexed testing, and experimental complexity which is intended to ensure that the 3D cell culture perfusion of organs-on-a-chip model into routine research tool adopted by academic and industry stakeholders.

To move the field forward, we present a scalable throughput organ-on-a-chip insert a tube system that requires 48 to operate the 3D cell culture models of organ independent statistician. Then, we introduce in-good perfusion to avoid the loss of cell signaling and drug metabolites in an otherwise one-way flow of perfusate. Furthermore, in order to improve the relevance of the model cell culture in 3D in vitro, we address the problem of transport of oxygen by the blood using, for the first time, an analog of hemoglobin breathing to increase gas deliveries cell respiration, as in vivo approximately 98% of the delivery of oxygen to cells occurs through reversible binding hemoglobin.

Next, we show that an increase in cellular metabolic pathways oxygenation shift towards oxidative phosphorylation that contribute to the maintenance of differentiated phenotype in vitro liver. Finally, we show that the activity of the cytochrome family of P450 enzymes of drug metabolism increased and prolonged in hepatocytes human primary cultured in 3D compared with two-dimensional (2D) cell culture gold standard with important consequences for drug metabolism, drug-drug interactions and pharmacokinetic studies in vitro.

Vasculogenesis of Human Dental Pulp Stem Cells Grown in Matrigel with Fully Defined Serum Free Culture Media

Generation of blood vessels is one of the most important challenges in tissue engineering and regeneration. Human cells pulp stem teeth (hDPSCs) are some of the most promising types of stem cells to induce vasculogenesis and angiogenesis because they not only secrete vascular endothelial growth factor (VEGF), but also can differentiate in vitro into both endotheliocytes and pericytes in culture serum-free media.

Additionally, hDPSCs can generate a complete blood vessel that contains both endothelial and mural coating in vivo after transplantation into adult brain. However, many serum-free media used for growth of a supplement containing hDPSCs undisclosed composition. It generates uncertainty as to which component of the appropriate necessary and dumped to the differentiation of vascular hDPSCs, and also block the transfer of basic research findings to clinical cell therapy.

In this work, we designed and tested a new medium that endothelial differentiation to the composition fully defined using standard basal culture medium plus B27 mixture, heparin and growth factors, including VEGF-A165 at different concentrations. We also optimized in vitro Matrigel assay to characterize both hDPSCs ability to differentiate cells of blood vessels and their capacity to produce tubular blood vessel in 3D culture.

Description of serum-free culture medium for the induction of vasculogenesis fully defined using human adult stem cells as a potential highlight relevant innovation for tissue engineering applications. In conclusion, we achieve efficient vasculogenesis start hDPSCs using serum-free culture medium with fully defined composition, which is valid for the purpose of human cell therapy.

Protection of human induced stem cells are pluripotent against shear stresses in suspension culture by Bingham plastic fluid

suspension cultures is an important method used in the industrial preparation of pluripotent stem cells (PSC), for regenerative therapy and drug screening. Generally, suspension culture require agitation to keep the PSC suspended aggregate and to promote mass transfer, but agitation also cause cell damage.

In this study, we investigated the use of Bingham plastic fluid, equipped with a polysaccharide-based polymer, to preserve the PSC of damage to cells in suspension culture. Rheometric analysis shows that the culture medium obtained yield stress and became Bingham plastic fluid, after supplementation with FP003 polymer.

Analysis of growth / death revealed that the formation of aggregates 2 days and 2 days of suspension in a Bingham plastic media improved cell growth and cell death is prevented. After the initial aggregation step, while the strong agitation (120 rpm) of the conventional culture medium resulted in massive cell death, in Bingham plastic fluid we obtain the same growth as normal culture with optimal agitation (90 rpm).

This shows that the Bingham plastic fluid cells are protected from shear stresses in suspension culture and can be used to increase their resilience when developing large scale. This article is protected by copyright. All rights reserved.

Co-Culture System Analysis Enteroids / Colonoids with innate Immune Cells

enteroids human intestine derived from adult stem cells offer the relevant ex vivo system for studying the biological process of the human gut. They create mobile and functional features of the intestinal epithelium of the small intestine (enteroids) or intestine (colonoids) although limited by the lack of relevant cell types that help maintain tissue homeostasis and to respond to external challenges.

In the intestine, innate immune cells interact with the function of epithelial barrier function support, and deploy effector. We have established a co-culture system enteroid / monolayers colonoid and macrophages underlying and polymorphonuclear neutrophils to recapitulate cellular skeleton of human intestinal epithelial niche. Enteroids generated from biopsies or tissue resected from every segment of the intestines of humans and maintained in long-term culture as a three-dimensional structure through supplementation stem cell growth factor. immune cells isolated from human whole blood fresh or frozen peripheral blood mononuclear cells (PBMC).

A 3D Cell Culture Organ-on-a-Chip Platform With a Breathable Hemoglobin Analogue Augments and Extends Primary Human Hepatocyte Functions in vitro

Monocytes from PBMC differentiated into macrophages by cytokine stimulation prior to co-culture. The method is divided into two main components of the model: (1) generate enteroid / monolayers colonoid and isolating immune cells and (2) the assembly enteroid / colonoid-immune cell co-culture with separate apical and basolateral compartment. Co-culture containing macrophages can be maintained for 48 hours while they neutrophils involved, because of their shorter life span, remain viable for 4 hours.

Enteroid co-cultures of immune-activating several outcome measures, including transepithelial resistance, production of cytokines / chemokines, phenotypic analysis of immune cells, immunofluorescence tissue imaging, protein or mRNA expression, antigen or microbial uptake, and other cellular functions. © 2020 Wiley Periodicals LLC.

Human Umbilical Cord Mesenchymal Stem Cells

HUXUC-01001 1*10^6/vial
EUR 1159

Human Umbilical Cord PrimaCell1: Normal Umbilical Artery Endothelial Cells

2-96106 1 Kit Ask for price

Human Umbilical Cord PrimaCell5: Normal Umbilical Vein Smooth Muscle Cells

2-96110 1 Kit Ask for price

Human Umbilical Cord PrimaCell2: Normal Umbilical Artery Smooth Muscle Cells

2-96107 1 Kit Ask for price

Human Umbilical Cord PrimaCell4: Normal Umbilical Vein Endothelial Cells Growth Medium

9-46109 5 x 100 ml Ask for price

Human Umbilical Cord PrimaCell1: Normal Umbilical Artery Endothelial Cells Growth Medium

9-46106 5 x 100 ml Ask for price

Human Umbilical Cord Mesenchymal Stem Cells With GFP

HUXUC-01101 1*10^6/vial
EUR 1599

Rat Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells

2-82587 1 Kit Ask for price

Mouse Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells

2-82091 1 Kit Ask for price

Human Umbilical Cord PrimaCell5: Normal Umbilical Vein Smooth Muscle Cells Growth Medium

9-46110 5 x 100 ml Ask for price

Human Umbilical Cord PrimaCell2: Normal Umbilical Artery Smooth Muscle Cells Growth Medium

9-46107 5 x 100 ml Ask for price

Human Umbilical Cord Tissue Preparation Buffer 4: Normal Umbilical Vein Endothelial Cells

9-80109 1 x 100 ml Ask for price

Human Umbilical Cord Tissue Preparation Buffer 1: Normal Umbilical Artery Endothelial Cells

9-80106 1 x 100 ml Ask for price

Human Umbilical Cord Tissue Preparation Buffer 5: Normal Umbilical Vein Smooth Muscle Cells

9-80110 1 x 100 ml Ask for price

Human Umbilical Cord Tissue Preparation Buffer 2: Normal Umbilical Artery Smooth Muscle Cells

9-80107 1 x 100 ml Ask for price

Human Primary Umbilical Cord-derived Mesenchymal Stem Cells

T5483 1x10^6 cells / 1.0 ml
EUR 1275

Complete Medium For Human Umbilical Cord Mesenchymal Stem Cells

HUXUC-90011 500mL
EUR 319

Rat Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Medium

9-25087 5 x 100 ml Ask for price

Mouse Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Medium

9-32091 5 x 100 ml Ask for price

Rat Umbilical Cord Tissue Preparation Buffer: Normal Umbilical Vein Smooth Muscle Cells

9-80294 1 x 100 ml Ask for price

Mouse Umbilical Cord Tissue Preparation Buffer: Normal Umbilical Vein Smooth Muscle Cells

9-80205 1 x 100 ml Ask for price

Serum Free Medium For Human Umbilical Cord Mesenchymal Stem Cells

HUXUC-90061 500mL
EUR 399

Immortalized Bovine Umbilical Cord Endothelial Cells (BUcEC)

T0834 1x10^6 cells / 1.0 ml Ask for price

Dog Primary Umbilical Cord-derived Mesenchymal Stem Cells

T5490 1x10^6 cells / 1.0 ml
EUR 1145

Dog Primary Umbilical Cord-derived Mesenchymal Stem Cells

T5491 1x10^6 cells / 1.0 ml
EUR 1145

Single Donor Human Umbilical Cord

IRHUSCD each
EUR 957
Description: Single Donor Human Umbilical Cord

Human Umbilical Cord PrimaCell1: Normal Umbilical Artery Endothelial Cells Growth Supplements with Serum (for 500 ml medium)

9-47106 1 Set Ask for price

Human Umbilical Cord PrimaCell4: Normal Umbilical Vein Endothelial Cells Growth Supplements with Serum (for 500 ml medium)

9-47109 1 Set Ask for price

Human Umbilical Cord PrimaCell5: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)

9-47110 1 Set Ask for price

Osteogenic Differentiation Medium For Human Umbilical Cord Mesenchymal Stem Cells

HUXUC-90021 200mL
EUR 250

Adipogenic Differentiation Medium For Human Umbilical Cord Mesenchymal Stem Cells

HUXUC-90031 200+100mL
EUR 359

Chondrogenic Differentiation Medium For Human Umbilical Cord Mesenchymal Stem Cells

HUXUC-90041 100mL
EUR 409

Human Umbilical Cord PrimaCell2: Normal Umbilical Artery Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)

9-47107 1 Set Ask for price

Fetal Umbilical Cord Lysate

XBL-10430 0.1 mg
EUR 500.1
Description: Fetal human umbilical cord tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The fetal human umbilical cord tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the umbilical cord tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The umbilical cord tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Human Umbilical Cord Fetal Tissue Lysate

IHUCDFSTL100UG each
EUR 870
Description: Human Umbilical Cord Fetal Tissue Lysate

Rat Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)

9-26087 1 Set Ask for price

Mouse Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)

9-33091 1 Set Ask for price

Human Umbilical Cord OptiTDS1: Tissue Dissociation System

4-28106 1 Kit Ask for price

Human Umbilical Cord OptiTDS2: Tissue Dissociation System

4-28107 1 Kit Ask for price

Human Umbilical Cord OptiTDS4: Tissue Dissociation System

4-28109 1 Kit Ask for price

Human Umbilical Cord OptiTDS5: Tissue Dissociation System

4-28110 1 Kit Ask for price

Human ; Umbilical Vein Endothelial Cells

02-701 100ng
EUR 481.2
Description: The Human ; Umbilical Vein Endothelial Cells is available in Europe and for worldwide shipping via Gentaur.

Human Umbilical Vein Endothelial Cells

HEC01 500,000+ Cells frozen
EUR 528

Human Umbilical Vein Endothelial Cells

ABC-TC3848 1 vial Ask for price
Description: HUVEC are isolated from the vein of the umbilical cord and are commonly used for physiological and pharmacological investigations, such as macromolecule transport, blood coagulation, angiogenesis, and fibrinolysis. Human umbilical vein endothelial cells have played a major role as a model system for the study of the regulation of endothelial cell function and the role of the endothelium in the response of the blood vessel wall to stretch, shear forces, and the development of atherosclerotic plaques.Each human umbilical cord vein is individually processed to isolate endothelial cells through collagenase digestion and culture. Frozen HUVEC products are cryopreserved at the end of the primary culture. HUVEC are guaranteed to reach 15 population doublings when cultured with Gentaur's recommended medium.Samples from each donor are tested via PCR to confirm non-reactivity.

Human Umbilical Vein Endothelial Cells

HUVEC-20001 1*10^6/vial
EUR 1100

OCPB00610-100UG - Fetal Umbilical Cord Lysate

OCPB00610-100UG 0.1mg
EUR 439

Human Umbilical Artery Endothelial Cells

ABC-TC3841 1 vial Ask for price
Description: Gentaur endothelial cells from large vessels are available from different locations, e.g. umbilical vein, umbilical artery, aorta, coronary artery, pulmonary artery, and saphenous vein. In addition, Gentaur offers HUVEC isolated in standard Endothelial Cell Growth Medium and also in Endothelial Cell Growth Medium 2. The cells are supplied either from single donors or from pooled donors (from up to four different umbilical cords). Furthermore, HUVEC are available which are prescreened for VEGF (vascular endothelial cell growth factor) response. Shortly after isolation, Gentaur Human Endothelial Cells from large vessels are cryopreserved at passage 1 or passage 2 (see page 5) using Gentaur's proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing.Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur.

Human Umbilical Vein Smooth Muscle Cells

ABC-TC3857 1 vial Ask for price
Description: Vascular smooth muscle cell is the cellular substate of most significant arterial disease. The increased growth potential of vascular smooth muscle cells represents one of the crucial anomalies responsible for the development of essential vascular diseases. New studies demonstrate that vascular smooth muscle cells express ICAM-1 and VCAM-1, which may contribute to the inflammatory reaction in the vascular wall and may actively be involved in the progression and stability of vascular disease. In vitro culture of human VSMC as a model of vascular research played a critical role and continues providing information in the pharmacology and the therapy of vascular diseases.HUVSMC from Gentaur Research Laboratories are isolated from human umbilical veins. HUVSMC are cryopreserved at the end of secondary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HUVSMC are characterized by immunofluorescent method with antibodies to ?-smooth muscle actin and desmin. HUVSMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HUVSMC are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Human Umbilical Artery Smooth Muscle Cells

ABC-TC3843 1 vial Ask for price
Description: Smooth muscle tissue is found in the tunica media layer of large and small blood vessels and in the walls of hollow organs like the bladder and the uterus. Smooth Muscle Cells (SMC) possess all the same types of filaments, but, depending on the tissue of origin, differ significantly in mechanical and physiological properties. Therefore, Gentaur offers a range of Human Smooth Muscle Cells produced at Gentaur's cell culture facility from normal human tissue of different origins. The cells are isolated from the aorta, the coronary artery, the pulmonary artery, the umbilical artery,the trachea, the bronchi, the bladder,the prostate, and the uterus. Shortly after isolation, all Gentaur Human Smooth Muscle Cells are cryopreserved at passage 2 (P2) using Gentaur's proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing.Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur.

Innovative Grade US Origin Bovine Umbilical Cord

IGBOCDF each
EUR 210
Description: Innovative Grade US Origin Bovine Umbilical Cord

Innovative Grade US Origin Bovine Umbilical Cord

IGBOCDP each
EUR 210
Description: Innovative Grade US Origin Bovine Umbilical Cord

Innovative Grade US Origin Bovine Umbilical Cord

IGBOCDS each
EUR 133
Description: Innovative Grade US Origin Bovine Umbilical Cord

Innovative Grade US Origin Bovine Umbilical Cord

IGBOCDZ each
EUR 133
Description: Innovative Grade US Origin Bovine Umbilical Cord

Innovative Grade US Origin Porcine Umbilical Cord

IGPCCDF each
EUR 133
Description: Innovative Grade US Origin Porcine Umbilical Cord

Innovative Grade US Origin Porcine Umbilical Cord

IGPCCDP each
EUR 210
Description: Innovative Grade US Origin Porcine Umbilical Cord

Innovative Grade US Origin Porcine Umbilical Cord

IGPCCDS each
EUR 210
Description: Innovative Grade US Origin Porcine Umbilical Cord

Innovative Grade US Origin Porcine Umbilical Cord

IGPCCDZ each
EUR 133
Description: Innovative Grade US Origin Porcine Umbilical Cord

Human Umbilical Vein Endothelial Cells, Pooled

ABC-TC3849 1 vial Ask for price
Description: The cells are cryopreserved in the second passage to ensure the highest viability and plating efficiency. Primary pooled HUVECs are derived from 10 individual donors, minimizing the lot-to-lot variability associated with cells derived from single donors.

HighQC™ RFP-Human Umbilical Cord-Derived Mesenchymal Stem Cell

ABC-FC0048 1 vial Ask for price
Description: HUcMSCs are isolated from umbilical cord matrix (Wharton's Jelly) and demonstrated with spindle-shaped-, and fibroblast-like cells. RFP-HUcMSCs are puromycin resistant HUcMSCs after trandsfected with lentiviruses expressing RFP under the control of CMV protomer. Each lot of RFP-HUcMSCs is tested to ensure the cells can be passaged at least three times (i.e., approximately 9 to 10 population doublings) after thaw in complete growth media (Mesenchymal Stem Cell Growth Medium (MSCGM)), when cultured following the detailed protocol described below.

Human Umbilical Vein Endothelial Cells, Neonatal

ABC-TC3853 1 vial Ask for price
Description: Human Umbilical Vein Endothelial Cells (HUVEC) are isolated from normal human umbilical vein. They are cryopreserved at the end of primary culture and can be cultured and propagated at least 16 population doublings.

Rat Umbilical Cord OptiTDS: Tissue Dissociation System

4-28294 1 Kit Ask for price

Human Primary Umbilical Vein Endothelial Cells

T4098 5x10^5 cells / 1.0 ml Ask for price

Human Primary Umbilical Vein Endothelial Cells

T5482 1x10^6 cells / 1.0 ml
EUR 765

Mouse Umbilical Cord OptiTDS: Tissue Dissociation System

4-28205 1 Kit Ask for price

Human Umbilical Artery Endothelial Cells, Neonatal

ABC-TC3842 1 vial Ask for price
Description: Human Umbilical Artery Endothelial Cells (HUAEC) are isolated from normal human umbilical arteries. They are cryopreserved at second passage and can be cultured and propagated at least 15 population doublings.

Human Primary Umbilical Artery Endothelial Cells

T4099 5x10^5 cells / 1.0 ml Ask for price

HighQC™ Human Mesenchymal Stem Cell (Umbilical Cord Matrix/ Cell Pellet In RNAlater)

ABC-SC0038T 1 vial Ask for price
Description: Mesenchymal Stem Cells (MSC), also termed Mesenchymal Stromal Cells, are multipotent cells that can differentiate into a variety of cell types and have the capacity for self renewal. MSC have been shown to differentiate in vitro or in vivo into adipocytes, chondrocytes, osteoblasts, myocytes, neurons, hepatocytes, and pancreatic islet cells. Optimized Gentaur media are available to support both the growth of MSC and their differentiation into several different lineages. Recent experiments suggest that differentiation capabilities into diverse cell types vary between MSC of different origin.Gentaur hMSC are harvested from normal human adipose tissue, bone marrow, and umbilical cord matrix (Wharton's jelly) of individual donors.The cells are tested for their ability to differentiate in vitro into adipocytes, chondrocytes, and osteoblasts. Our hMSC show a verified marker expression profile that complies with ISCT* recommendations, providing well characterized cells.

Human Umbilical Vein Smooth Muscle Cells, Neonatal

ABC-TC3858 1 vial Ask for price
Description: Human umbilical vein smooth muscle cells (HUVSMC) are derived from the umbilical vein. They are cryopreserved at second passage. HUVSMC can be cultured and propagated 6 population doublings.

Human Primary Umbilical Vein Smooth Muscle Cells

T4101 5x10^5 cells / 1.0 ml
EUR 1375

Human Umbilical Vein Endothelial Cells, Pooled Donor

ABC-TC3854 1 vial Ask for price
Description: HUVEC-Umbilical Vein Endothelial Cells are available as single donor or pooled varieties.

Human Umbilical Vein Endothelial Cells, Single Donor

ABC-TC3855 1 vial Ask for price
Description: Gentaur endothelial cells from large vessels are available from different locations, e.g. umbilical vein, umbilical artery, aorta, coronary artery, pulmonary artery, and saphenous vein. In addition, Gentaur offers HUVEC isolated in standard Endothelial Cell Growth Medium and also in Endothelial Cell Growth Medium 2. The cells are supplied either from single donors or from pooled donors (from up to four different umbilical cords). Furthermore, HUVEC are available which are prescreened for VEGF (vascular endothelial cell growth factor) response. Shortly after isolation, Gentaur Human Endothelial Cells from large vessels are cryopreserved at passage 1 or passage 2 (see page 5) using Gentaur's proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing.Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur.

Human Umbilical Artery Smooth Muscle Cells, Neonatal

ABC-TC4269 1 vial Ask for price
Description: Human Umbilical Artery Smooth Muscle Cells (HUASMC) are isolated from normal human umbilical arteries. They are cryopreserved at second passage and can be cultured and propagated at least 16 population doublings. Umbilical artery smooth muscle cells have

Human Primary Umbilical Artery Smooth Muscle Cells

T4100 5x10^5 cells / 1.0 ml Ask for price

GFP Expressing Human Umbilical Vein Endothelial Cells

GF01 500,000+ Cells frozen
EUR 1248

Human Umbilical Vein Endothelial Cells, Pre-screened

ABC-TC3850 1 vial Ask for price
Description: Gentaur endothelial cells from large vessels are available from different locations, e.g. umbilical vein, umbilical artery, aorta, coronary artery, pulmonary artery, and saphenous vein. In addition, Gentaur offers HUVEC isolated in standard Endothelial Cell Growth Medium and also in Endothelial Cell Growth Medium 2. The cells are supplied either from single donors or from pooled donors (from up to four different umbilical cords). Furthermore, HUVEC are available which are prescreened for VEGF (vascular endothelial cell growth factor) response. Shortly after isolation, Gentaur Human Endothelial Cells from large vessels are cryopreserved at passage 1 or passage 2 (see page 5) using Gentaur's proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing.Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur.

Human Umbilical Cord FibrOut1: Fibroblast Inhibitory System (for 500 ml medium)

7-15095 1 Kit Ask for price

Human Umbilical Cord FibrOut2: Fibroblast Inhibitory System (for 500 ml medium)

7-15096 1 Kit Ask for price

Human Umbilical Cord FibrOut4: Fibroblast Inhibitory System (for 500 ml medium)

7-15098 1 Kit Ask for price

Human Umbilical Cord FibrOut5: Fibroblast Inhibitory System (for 500 ml medium)

7-15099 1 Kit Ask for price

GFP-Human Umbilical Vein Endothelial Cells

ABC-FC0001 1 vial Ask for price
Description: HUVECs were infected with GFP- expressing Lentiviral particles @ passage 1. Puromycin resistant GFP-HUVECs were selected and shipped in frozen vials (cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum of 18 population doubling levels when cultured following the protocols described below.

RFP-Human Umbilical Vein Endothelial Cells

ABC-FC0004 1 vial Ask for price
Description: HUVECs were infected with RFP- expressing Lentiviral particles @ passage 1. Zeocin resistant RPF-HUVECs were selected and shipped in frozen vials (cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum of 18 population doubling levels when cultured following the protocols described below).

Human Cord Blood CD4+ Helper T Cells

ABC-TC3376 1 vial Ask for price
Description: Cord Blood CD4+ Helper T cells are negatively isolated from mononuclear cells using an indirect immunomagnetic CD4+ T cell labeling system to deplete the non-CD4+ cells.

GFP-Human Umbilical Artery Endothelial Cells

ABC-FC0021 1 vial Ask for price
Description: HUAECs are pooled cells isolated from normal human umbilical artery. GFP-HUAECs were selected from HUAECs infected with GFP-expressing lentiviruse with Puromycin and the cells are shipped in frozen vials (the cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 20 when cultured following the detailed protocol.

RFP-Human Umbilical Artery Endothelial Cells

ABC-FC0022 1 vial Ask for price
Description: HUAECs are pooled cells isolated from normal human umbilical artery. RFP-HUAECs were selected from HUAECs infected with RFP-expressing lentiviruses with Zyocin and the cells are shipped in frozen vials (the cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 20 when cultured following the detailed protocol.

Immortalized Human Umbilical Vein Endothelial Cells - SV40

T0014 1x106 cells / 1.0 ml Ask for price

Human Umbilical Vein Endothelial Cells, 10-Donor Pool

ABC-TC3852 1 vial Ask for price
Description: HUVEC10-Donor Pools, when grown in LIVas Medium, provide an ideal low-serum culture model, with or without human VEGF, for the study of angiogenesis, atherosclerosis or vascular biology without the concern of atypical results from a single donor. HUVEC 10-Donor Pools are cryopreserved as secondary cells to ensure the highest viability and plating efficiency.Cell Features:HUVEC are isolated from human umbilical cords, cultured on plastic and cryopreserved as primary cells.HAoEC and HUVEC 10-Donor Pool are isolated from human aorta or umbilical cord, cultured on plastic and cryopreserved as secondary cells.HPAEC are isolated from human pulmonary artery, cultured on plastic and cryopreserved as secondary cells.HCAEC are isolated from human coronary arteries, cultured on plastic and cryopreserved as tertiary cells.Human endothelial cells can be grown in low serum (2%) medium without phenol red or antimicrobials when cultured in VascuLife medium.Culture human endothelial cells with or without VEGF. human endothelial cells are extensively tested to meet quality standards and exhibit optimal performance.Gentaur guarantees performance and quality.

Complete Medium For Human Umbilical Vein Endothelial Cells

HUVEC-90011 500mL
EUR 319

Mitochondria GFP Tag Human Umbilical Vein Endothelial Cells

ABC-FC0002 1 vial Ask for price
Description: HUVECs were infected with Lentiviral particles expressing GFP-Tagged Cytochrome C oxidase VIII @ passage 1. Puromycin resistant Mitochondria-GFP-HUVECs were selected and shipped in frozen vials (cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum of 18 population doubling levels when cultured following the protocols described below).

CORNING®HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS, HUVEC-2 CELLS (>5X105 CELLS), 1 CRYOVIAL/PACK

354151 1/pk
EUR 465.6
Description: Permeable Support - DL; Coated Transwells - DL

Immortalized Human Umbilical Vein Endothelial Cells (PDL8 HUVEC)

T0725 1x10^6 cells / 1.0 ml
EUR 1085

human umbilical vein endothelial cells, Peptide Aptamer, unlabeled

AP-316-U 5 mg Ask for price

Human Pre-screened Umbilical Vein Endothelial Cells, Neonatal

ABC-TC3763 1 vial Ask for price
Description: Pre-screened for endothelial properties. Human Umbilical Vein Endothelial Cells (HUVEC) are isolated from normal human umbilical vein. They are cryopreserved at the end of primary culture and can be cultured and propagated at least 16 population doubling.

Human Cord Blood CD4+/ CD45RA+ Naive T Cells

CBCD4-45RA-C10M 10 million
EUR 1378.8

Human Cord Blood CD4+/ CD45RA+ Naive T Cells

CBCD4-45RA-C15M 15 million
EUR 1678.8

Human Cord Blood CD4+/ CD45RA+ Naive T Cells

CBCD4-45RA-C5M 5 million
EUR 838.8

Human Cord Blood CD4+/ CD45RA+ Naive T Cells

CBCD4-45RA-F10M 10 million
EUR 1798.8

Human Cord Blood CD4+/ CD45RA+ Naive T Cells

CBCD4-45RA-F15M 15 million
EUR 1980

Human Cord Blood CD4+/ CD45RA+ Naive T Cells

CBCD4-45RA-F5M 5 million
EUR 1198.8

Human Cord Blood CD4+/CD45RA+ Naïve T Cells

ABC-TC3377 1 vial Ask for price
Description: First, Cord Blood-CD4+ T Cells are negatively isolated using an indirect immunomagnetic CD4+ labeling system from mononuclear cells. Next, CD45RO MicroBeads are used to deplete the CD45RO+ population, leaving a purified CD4+/CD45RA+ Naïve T Cell population.

Plasma Membrane GFP Tag Human Umbilical Vein Endothelial Cells

ABC-FC0003 1 vial Ask for price
Description: HUVECs were infected with Lentiviral particles expressing GFP-Tagged neuromodulin @ passage 1. Puromycin resistant PM-GFP-HUVECs were selected and shipped in frozen vials (cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum of 18 population doubling levels when cultured following the protocols described below).

human umbilical vein endothelial cells, Peptide Aptamer, Biotinylated

AP-316-B 1 mg Ask for price

Rat Umbilical Cord FibrOut: Fibroblast Inhibitory System (for 500 ml medium)

7-15264 1 Kit Ask for price

human umbilical vein endothelial cells, Peptide Aptamer, FITC labelled

AP-316-F 1 mg Ask for price

Mouse Umbilical Cord FibrOut: Fibroblast Inhibitory System (for 500 ml medium)

7-15184 1 Kit Ask for price

Human Cord Blood Naive T Helper Cells, CD4+

ABC-TC3970 1 vial Ask for price
Description: Human Peripheral Blood Mononuclear Cells are available as positive and negative controls for T-cell monitoring in ELISPOT, ELISA, cytokine bead array, tetramer/pentamer, and flow cytometry assays. A peripheral blood mononuclear cell is defined as any blood cell with a round nucleus. These blood cells are a critical component in the immune system to fight infection and adapt to intruders. The lymphocyte population consists of CD4+ and CD8+ T cells, B cells and Natural Killer cells, CD14+ Monocytes, and Basophils/Neutrophils/Eosinophils/Dendritic cells. These cells are often extracted from whole blood or from leukopacks using ficoll, a hydrophilic polysaccharide that separates layers of blood, with monocytes and lymphocytes forming a buffy coat under a layer of plasma.Samples from each donor are tested via PCR to confirm non-reactivity.

Human Cord Blood T-Cells, CD4+

ABC-TC3971 1 vial Ask for price
Description: Human Peripheral Blood Mononuclear Cells are available as positive and negative controls for T-cell monitoring in ELISPOT, ELISA, cytokine bead array, tetramer/pentamer, and flow cytometry assays. A peripheral blood mononuclear cell is defined as any blood cell with a round nucleus. These blood cells are a critical component in the immune system to fight infection and adapt to intruders. The lymphocyte population consists of CD4+ and CD8+ T cells, B cells and Natural Killer cells, CD14+ Monocytes, and Basophils/Neutrophils/Eosinophils/Dendritic cells. These cells are often extracted from whole blood or from leukopacks using ficoll, a hydrophilic polysaccharide that separates layers of blood, with monocytes and lymphocytes forming a buffy coat under a layer of plasma.Samples from each donor are tested via PCR to confirm non-reactivity.

VEGF-stimulated human umbilical vein endothelial cells, Peptide Aptamer, unlabeled

AP-339-U 5 mg Ask for price

VEGF-stimulated human umbilical vein endothelial cells, Peptide Aptamer, Biotinylated

AP-339-B 1 mg Ask for price

VEGF-stimulated human umbilical vein endothelial cells, Peptide Aptamer, FITC labelled

AP-339-F 1 mg Ask for price

Human Cord Blood CD3+ Pan T Cells

CBCD3-C10M 10 million
EUR 1020

Human Cord Blood CD3+ Pan T Cells

CBCD3-C20M 20 million
EUR 1560

Human Cord Blood CD3+ Pan T Cells

CBCD3-F10M 10 million
EUR 1068

Human Cord Blood CD3+ Pan T Cells

CBCD3-F20M 20 million
EUR 1800

Human Cord Blood Pan T Cells

ABC-TC3385 1 vial Ask for price
Description: Cord Blood-Pan T cells are negatively isolated from mononuclear cells, using an indirect immunomagnetic Pan T labeling system.

Human Umbilical Vein Endothelial

ABC-TC3997 1 vial Ask for price
Description: Gentaur's normal Human Umbilical Vein Endothelial Cells (HUVEC), when grown in Gentaur's LIVas Medium, provides an ideal low serum culture model, with or without human VEGF, for the study of angiogenesis, atherosclerosis or vascular biology. Gentaur's HUVEC are cryopreserved as primary cells to ensure the highest viability and plating efficiency. Our HUVEC are quality tested in LIVas 2% serum medium to ensure optimal reduced-serum growth over a period of at least 15 population doublings at rates equal to or greater than serum-supplemented medium. Cell Features:HUVEC are isolated from human umbilical cords, cultured on plastic and cryopreserved as primary cells.HAoEC and HUVEC 10-Donor Pool are isolated from human aorta or umbilical cord, cultured on plastic and cryopreserved as secondary cells.HPAEC are isolated from human pulmonary artery, cultured on plastic and cryopreserved as secondary cells.HCAEC are isolated from human coronary arteries, cultured on plastic and cryopreserved as tertiary cells.Human endothelial cells can be grown in low serum (2%) medium without phenol red or antimicrobials when cultured in VascuLife medium.Culture human endothelial cells with or without VEGF.Gentaur's human endothelial cells are extensively tested to meet quality standards and exhibit optimal performance.Gentaur guarantees performance and quality.

Human Cord Blood CD8+ Cytotoxic T Cells

CBCD8-C5M 5 million
EUR 958.8

Human Cord Blood CD8+ Cytotoxic T Cells

CBCD8-F5M 5 million
EUR 1078.8

Human Cord Blood CD8+ Cytotoxic T Cells

ABC-TC3379 1 vial Ask for price
Description: Cord Blood-CD8+ Cytotoxic T cells are negatively isolated from mononuclear cells, using an indirect immunomagnetic CD8 labeling system to deplete the non-CD8+ cells.

Immortalized Human Umbilical Vein Endothelial Cell Line

ABI-TC051D 1 vial Ask for price
Description: The endothelium is the thin layer of cells that lines the interior surface of blood vessels and lymphatic vessels, forming an interface between circulating blood or lymph in the lumen and the rest of the vessel wall. Gentaur currently offers human endothelial cells isolated from the vein of the human umbilical cord (HUVEC).

Human Cord Blood CD19+ B Cells

CBCD19-C2.5M 2.5 million
EUR 906

Human Cord Blood CD19+ B Cells

CBCD19-C5M 5 million
EUR 1078.8

Human Cord Blood CD19+ B Cells

CBCD19-F5M 5 million
EUR 1198.8

Human Cord Blood CD56+ Nk Cells

CBCD56-C1M 1 million
EUR 886.8

Human Cord Blood CD56+ Nk Cells

CBCD56-F1M 1 million
EUR 994.8

Human Cord Blood CD19+ B Cells

ABC-TC3368 1 vial Ask for price
Description: Cord Blood-CD19+ B cells are positively isolated from mononuclear cells, using a direct immunomagnetic CD19 MicroBeads labeling system

Human Cord Blood CD56+ NK Cells

ABC-TC3378 1 vial Ask for price
Description: Cord Blood CD56+ Natural Killer (NK) Cells are positively isolated from Mononuclear Cells using a direct immunomagnetic CD56 MicroBeads labeling system.

Human Cord Blood CD34+ Cells, Mixed

ABC-TC3369 1 vial Ask for price
Description: Cord Blood-CD34+ Stem/Progenitor cells are positively isolated using a direct immunomagnetic CD34 MicroBead labeling system.Cord Blood-CD34+ Cells/Mixed indicates that multiple donors are pooled to obtain the cord blood CD34+ cells.

Human Cord Blood CD133+ Cells, Mixed

ABC-TC3366 1 vial Ask for price
Description: Cord Blood-CD133+ Stem/Progenitor cells are positively isolated using a direct immunomagnetic CD133 MicroBead labeling system. The majority of CD133+ stem/progenitor cells express the CD34 cell marker as well. Cord Blood-CD133+ Cells/Mixed indicates that multiple donors are used to obtain the cord blood CD133+ cells

Human Cord Blood CD34+ Cells, Single

ABC-TC3371 1 vial Ask for price
Description: Cord Blood-CD34+ Stem/Progenitor cells are positively isolated using a direct immunomagnetic CD34 MicroBead labeling system. Cord Blood-CD34+ Cells/Single indicates that only one donor is used to obtain the cord blood CD34+ cells.

Human Cord Blood CD34+ Cells Derived Endothelial Cells

HEC23 500,000+ Cells
EUR 1296

Human Cord Blood-CD133+ Cells, Single

ABC-TC3387 1 vial Ask for price
Description: Cord Blood-CD133+ Stem/Progenitor cells are positively isolated using a direct immunomagnetic CD133 MicroBead labeling system. The majority of CD133+ stem/progenitor cells express the CD34 cell marker as well. Cord Blood-CD133+ Cells/Single indicates that only one donor is used to obtain the cord blood CD133+ cells.

Human Cord Blood CD34+ Stem / Progenitor Cells

CBCD34-deplete 1-2x10^8
EUR 718.8

Human Cord Blood CD34+ Stem / Progenitor Cells

CBCD34-F1M 1 million
EUR 2580

Human Cord Blood CD34+ Stem / Progenitor Cells

CBCD34mix-F1M 1 million
EUR 1980

Human Cord Blood CD34+ Progenitor Cells, Pooled

ABC-TC3576 1 vial Ask for price
Description: CD34 is a well-known marker for primitive and bone marrow-derived progenitor cells, especially for hematopoietic and endothelial progenitors. Gentaur offers CD34+ progenitor cells in a phenotypically undifferentiated state. The progenitor cells are derived from cord blood of healthy donors and produced at the Gentaur facility. Progenitor cells contain two main cellular subpopulations, hematopoietic and endothelial progenitor cells. CD34+ progenitor cells are suitable for a series of studies for directed differentiation into more committed types of blood cells and endothelial lineages. Immediately after isolation, the freshly prepared CD34+ progenitor cells are cryopreserved using a serum-free freezing medium.Samples from each donor are tested via PCR to confirm non-reactivity.Cord blood CD133+ cells are isolated using direct positive immunomagnetic selection for CD133+ cells. The majority of CD133+ cells also express CD34. CD133 is a glycoprotein with sub-cellular localization in plasma membrane protrusions. In humans, this protein was originally identified as an antigenic marker expressed on hematopoietic stem cells. In addition, these cells are highly proliferative primitive hematopoietic progenitors. They can give rise to mature terminally differentiated hematopoietic cells by means of directed differentiation.Gentaur offers CD133 cells in a phenotypically undifferentiated state. The cells are derived from fresh cord blood of healthy donors and produced at the Gentaur facility. Immediately after isolation, the freshly prepared CD133 cells are cryopreserved using a serum-based cryopreservation medium. Each vial contains 100,000 cells per vial.

VEGF-stimulated Human Umbilical Vein Endothelial Cell, Peptide Aptamer, unlabeled

AP-338-U 5 mg Ask for price

VEGF-stimulated Human Umbilical Vein Endothelial Cell, Peptide Aptamer, Biotinylated

AP-338-B 1 mg Ask for price

VEGF-stimulated Human Umbilical Vein Endothelial Cell, Peptide Aptamer, FITC labelled

AP-338-F 1 mg Ask for price

Human Cord Blood Mononuclear Cells CD133+ Depleted

ABC-TC3383 1 vial Ask for price
Description: Cord Blood-CD133-Depleted Mononuclear Cells are isolated by using a direct immunomagnetic CD133 MicroBead labeling system to deplete the CD133+ cells. This product contains only a minimal amount of Cord Blood-CD133+ cells.

Human Cord Blood CD34+ Cells, Cryopreserved, single donor

CBCD34-C0.5M - Ask for price

Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor 

CBCD34mix-C0.5M - Ask for price

Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor 

CBCD34mix-C1M - Ask for price

Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor 

CBCD34mix-C5M - Ask for price

Human Cord Blood CD34+ Cells, Cryopreserved, single donor 

CBCD34-C1M - Ask for price

Human Cord Blood CD36+ Early Erythroid Progenitor Cells

ABC-TC3375 1 vial Ask for price
Description: Cord Blood-CD36+ Early Erythroid Progenitor cells are derived from Cord Blood-CD34+ cells in culture. Cord Blood-CD34+ cells are cultured for seven to eight days using a serum free expansion media for hematopoietic cells, EPO (3U/ml), SCF (50ng/ml), IL-3 (10ng/ml), and IL-6 (10ng/ml) growth factors. The cells are harvested from culture, and the Cord Blood-CD36+ cells are positively isolated using a direct immunomagnetic CD36 MicroBead labeling system.

Human Peripheral Blood CD4+ T Cells

ABC-TC3315 1 vial Ask for price
Description: T Cells have a key function in the adaptive immune system. They can either promote growth and differentiation of other immune cells or show suppressive function and down-regulate immune reactions. These cells are often extracted from whole blood or from leukopacks using ficoll, a hydrophilic polysaccharide that separates layers of blood, with monocytes and lymphocytes forming a buffy coat under a layer of plasma. Samples from each donor are tested via PCR to confirm non-reactivity.

Human Peripheral Blood CD4+ T Cells

ABC-TC3993 1 vial Ask for price
Description: Human Peripheral Blood Mononuclear Cells are available as positive and negative controls for T-cell monitoring in ELISPOT, ELISA, cytokine bead array, tetramer/pentamer, and flow cytometry assays.

Human Cord Blood Mononuclear Cells (CB MNC)

CBMNC-C10M 10 million
EUR 166.8

Human Cord Blood Mononuclear Cells (CB MNC)

CBMNC-C150M 150 million
EUR 1066.8

Human Cord Blood Mononuclear Cells (CB MNC)

CBMNC-C50M 50 million
EUR 718.8

Human Cord Blood Mononuclear Cells (CB MNC)

CBMNC-F100M 100 million
EUR 1438.8

Human Cord Blood Mononuclear Cells (CB MNC)

CBMNC-F200M 200 million
EUR 2038.8

Human Cord Blood Mononuclear Cells (CB MNC)

CBMNC-F50M 50 million
EUR 958.8

HighQC™ Human Cord Blood CD34/CD133+ Progenitor Cells

ABC-SC0084T 1 vial Ask for price
Description: CD34 is a well-known marker for primitive and bone marrow-derived progenitor cells, especially for hematopoietic and endothelial progenitors. Gentaur offers CD34+ progenitor cells in a phenotypically undifferentiated state. The progenitor cells are derived from cord blood of healthy donors and produced at the Gentaur facility. Progenitor cells contain two main cellular subpopulations, hematopoietic and endothelial progenitor cells. CD34+ progenitor cells are suitable for a series of studies for directed differentiation into more committed types of blood cells and endothelial lineages. Immediately after isolation, the freshly prepared CD34+ progenitor cells are cryopreserved using a serum-free freezing medium.Samples from each donor are tested via PCR to confirm non-reactivity.Cord blood CD133+ cells are isolated using direct positive immunomagnetic selection for CD133+ cells. The majority of CD133+ cells also express CD34. CD133 is a glycoprotein with sub-cellular localization in plasma membrane protrusions. In humans, this protein was originally identified as an antigenic marker expressed on hematopoietic stem cells. In addition, these cells are highly proliferative primitive hematopoietic progenitors. They can give rise to mature terminally differentiated hematopoietic cells by means of directed differentiation.Gentaur offers CD133 cells in a phenotypically undifferentiated state. The cells are derived from fresh cord blood of healthy donors and produced at the Gentaur facility. Immediately after isolation, the freshly prepared CD133 cells are cryopreserved using a serum-based cryopreservation medium. Each vial contains 100,000 cells per vial.

Human CD133+ Progenitor Cells from Cord Blood, Pooled

ABC-TC4279 1 vial Ask for price
Description: CD133 (Prominin-1) is a glycoprotein with sub-cellular localization in plasma membrane protrusions. In humans, this protein was originally identified as an antigenic marker expressed on hematopoietic stem cells. In addition,CD133 is also expressed by neural and muscle progenitor cells. CD133+ cells are isolated in Gentaur's facilities from fresh cord blood. These cells are highly proliferative primitive progenitors. They can give rise to mature, terminally differentiated blood cells by means of directed differentiation. Gentaur also provides suitable expansion and differentiation media. Immediately after isolation, the freshly prepared CD133+ Progenitor Cells are cryopreserved using Gentaur's proprietary serum-free,animal component-free and defined freezing medium Cryo-SFM. Each cryo vial contains more than 100.000 viable cells after thawing.

HighQC™ Human Cord Blood CD34+ Stem/Progenitor Cells

ABC-SC0097T 1 vial Ask for price
Description: Cord blood CD34+ stem cells are isolated using positive immunomagnetic cell separation procedures from umbilical cord blood

HighQC™ Human IPSC From Cord Blood Cells CD34+

ABC-SC2028 1 vial Ask for price
Description: iPSC lines are derived using a standardized integration-free, episomal reprogramming protocols from CD34+ cord blood cells.

Human Cord Blood Mononuclear Cells CD34+ Depleted, Single

ABC-TC3384 1 vial Ask for price
Description: Cord Blood CD34 Depleted Mononuclear Cells are isolated, using a direct immunomagnetic CD34 MicroBead labeling system to deplete the CD34+ cells. This product contains only a minimal amount of CD34+ cells.Cord Blood-CD34 Depleted Mononuclear Cells/Single indicates that only one donor is used to obtain the cord blood mononuclear cells.

Human CD133+ Progenitor Cells from Cord Blood, Single Donor

ABC-TC4280 1 vial Ask for price
Description: CD133 (Prominin-1) is a glycoprotein with sub-cellular localization in plasma membrane protrusions. In humans, this protein was originally identified as an antigenic marker expressed on hematopoietic stem cells. In addition,CD133 is also expressed by neural and muscle progenitor cells. CD133+ cells are isolated in Gentaur's facilities from fresh cord blood. These cells are highly proliferative primitive progenitors. They can give rise to mature, terminally differentiated blood cells by means of directed differentiation. Gentaur also provides suitable expansion and differentiation media. Immediately after isolation, the freshly prepared CD133+ Progenitor Cells are cryopreserved using Gentaur's proprietary serum-free,animal component-free and defined freezing medium Cryo-SFM. Each cryo vial contains more than 100.000 viable cells after thawing.

Human CD34+ Progenitor Cells from Cord Blood, Single Donor

ABC-TC4282 1 vial Ask for price
Description: CD34 is a glycosylated transmembrane protein and represents a well-known marker for primitive blood- and bone marrow-derived progenitor cells, especially for hematopoietic and endothelial progenitors. Although the biological functions of CD34 are largely unknown,recent data suggest that CD34 is involved in maintenance of the progenitor cells in a phenotypically undifferentiated state. Gentaur offers CD34+ Progenitor Cells from the cord blood of healthy donors, produced at Gentaur's cell culture facility. The CD34+ Progenitor Cells contain two main cellular subpopulations, hematopoietic and endothelial progenitor cells. Therefore, CD34+ Progenitor Cells are suitable for a series of studies, e.g. directed differentiation into more committed types of blood cells and endothelial lineages.Immediately after isolation, the freshly prepared CD34+ Progenitor Cells are cryopreserved using Gentaur's proprietary serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 100,000 viable cells after thawing.

HighQC™ Cord Blood Mononuclear Cells

ABC-SC0128T 1 vial Ask for price
Description: Human cord blood mononuclear cells are separated from umbilical cord blood by means of a density gradient centrifugation protocol. MNCs are screened for the expression CD45 and checked for viability using flow cytometry prior to cryopreservation.

Human  Cord  Blood  CD34+  Cells (Mixed  Donors)

CBCD34M-05 - Ask for price

Human  Cord  Blood  CD34+  Cells (Mixed  Donors)

CBCD34M-1 - Ask for price

Human  Cord  Blood  CD34+  Cells (Mixed  Donors)

CBCD34M-5 - Ask for price

Human Mononuclear Cells from Cord Blood, Pooled, Ultra-pure

ABC-TC3713 1 vial Ask for price
Description: Gentaur Mononuclear Cells (MNC) represent the enriched lymphocyte and monocyte fraction of whole blood. They are isolated from umbilical cord blood and adult peripheral blood of healthy donors at Gentaur's cell culture facility. Mononuclear Cells from cord blood contain a relatively high percentage of primitive progenitor cells,whereas Mononuclear Cells from adult peripheral blood (hMNC-PB) contain large numbers of mature immune cells.Note: When culturing pooled hMNC-PB, a bidirectional allogeneic mixed lymphocyte reaction (MLR) is likely to occur during the first 48 hours. This will result in cell activation, blast transformation and T-cell proliferation. Thus, these cells are preferably used for several types of infectious virus tests, including neutralization assays. For HIV-related research, prior CD8+ T-cell depletion is recommended.Freshly collected whole blood is carefully separated by optimized low-density gradient centrifugation, effectively removing the granulocytes. In order to avoid general cell damage, red blood cells are gently depleted by proprietary techniques instead of using classic osmotic lysis protocols. The obtained ultra-pure Gentaur Mononuclear Cells do not clump after thawing, and they exhibit superior viability and unchanged biological function.Immediately after isolation, the freshly prepared Mononuclear Cells are cryopreserved using Gentaur's proprietary,serum-free freezing medium, Cryo-SFM.Each cryo vial contains more than 25 million viable cells after thawing.

Human Peripheral Blood CD4+ Naive T Helper Cells

ABC-TC3988 1 vial Ask for price
Description: Human Peripheral Blood Mononuclear Cells are available as positive and negative controls for T-cell monitoring in ELISPOT, ELISA, cytokine bead array, tetramer/pentamer, and flow cytometry assays.

Basic protocol 1: Nurseries fragment enteroid to Transwells for forming monolayer Alternative Protocol: Nurseries fragment enteroid for forming monolayer using trituration Protocol base 2: Isolation of monocytes, and derivation of immune cells from human peripheral blood basis of Protocol 3: Isolation of neutrophils from human peripheral blood IP base 4: Assembly enteroid / macrophages or enteroid / neutrophil co-culture.